Objective To analyze the feature of epidemiological of rotavirus diarrhea in Lulong county, Hebei province. Methods 426 stool specimens were collected from inpatant with acute diarrhea from children less than 5 years old. Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Results Rotavirus was detected in 202 of 426 (47.42%) specimens. Genotyping of rotavirus showed that G3 was predominant (57.9%), followed by Gmix(16.3%) ,G9(14.9%), G1 (7.9%), G4(1%), G2 (0.5%), P-genotyping showed that P[8], Pmix, P[4], P[9], type were found in 58.4% , 28.7% , 6.9% and 1% respectively. The most common G/P combination identified was G3P [8]. Conclusion Group A rotaviruses was a major pathogen of diarrhea in Children in Lulong. G3P[8] was the predominant type in 2009, Gmix and Pmix abound, and G9 serotypes has become the second predominant after G3 strain in the region.%目的 了解河北省轮状病毒腹泻的流行情况.方法 2008年9月至2009年8月收集卢龙县儿童医院及妇幼保健院5岁以下腹泻住院患儿标本共426份,采用酶联免疫吸附试验(ELISA)检测轮状病毒抗原,阳性标本用反转录-聚合酶链反应(RT-PCR)进行基因分型.结果 426份标本中轮状病毒检出率为47.42%(202/426).对202份轮状病毒阳性标本进行G/P分型,G3型是主要优势株,占57.9%(117/202),其次为混合G型感染(16.3%),G9型(14.9%),G1型(7.9%),G4型(1%),G2型(0.5%),P基因型最常见的为P[8]占58.4%,其次为P型混合感染占28.7%,P[4](6.9%),P[9](1%),最常见的G/P组合为G3P[8].结论 轮状病毒是卢龙地区儿童腹泻住院的主要病原,G3P[8]为主要流行株,混合型在该地区多发,且G9血清型在该地区已经成为仅次于G3的第二大优势株.
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