喉鳞状细胞癌患者人乳头瘤病毒感染的检测分析

摘要

Objective To analyze the infection of human papillomavirus in laryngeal squamous cell carcinoma patients.Methods The pathological samples of 64 clinical diagnosed laryngeal squamous cell carcinoma patients were collected.Lunimex and PCR techniques were used to detect the HPV gene infection and immunohistochemistry method was used to analyze the HPV protein expression in the samples.Results In the 64 cases,7 were positive for HPV infection by Luminex and PCR tests.18 were positive for HPV16/18 E6 protein expression.The total positive rate was about 39.1％.Conclusion The high HPV infection rate in laryngeal squamous cell carcinoma patients in the study indicated indirectly that the importance of the HPV infection in pathogenesis of the laryngeal squamous cell carcinoma.%目的 了解喉鳞状细胞癌患者人乳头瘤病毒感染的情况.方法 对收集到的64例临床诊断喉癌病例的石蜡组织标本应用Luminex及PCR的方法对HPV感染进行基因检测,应用免疫组织化学的方法对HPV16/18E6蛋白进行检测.结果 在64例临床诊断的喉癌病例中,通过Luminex及PCR的方法发现7例病例具有HPV的感染；通过免疫组织化学的方法发现18例病例具有HPV16/18的感染,基因及蛋白检测的总阳性率达到39.1％.结论 此研究发现在喉癌患者中较高的HPV感染率间接说明HPV感染对喉癌发生的重要性,为阐明喉癌的发病机制奠定基础.