SENP1在人神经胶质瘤细胞中的重要作用

摘要

Objective To investigate the role of sentrin/SUMO-specific protease 1 (SENP1) plays in human glioma cells by knockdown SENP1 with adenovirus transfection.Methods To knockdown SENP1,sh-SENP1 adenovirus were transfected into glioma cells LN229.The relative cell viability and apoptosis in glioma cells were observed to define the effect of SENP1 down-regulation on cell proliferation and apoptosis.And the effect of SENP1 down-regulation on cell migration was detected by a transwell migration system.Results SENP1 knockdown inhibited cell proliferation and induced apoptosis in human glioma cells.Further,we found that SENP1 knockdown obviously suppressed tumor cell migration.Conclusions These results demonstrate that SENP1 plays a critical role in human glioma cells.%目的 探讨小泛素相关修饰物(SUMO)特异性蛋白酶-1(SENP1)对神经胶质瘤细胞的生长和侵袭能力的影响.方法 采用腺病毒感染方法敲除胶质瘤细胞LN229的SENP1基因,然后用双染法检测基因敲除后对细胞增殖和细胞凋亡的影响,并用Tanswell小室法检测基因敲除后胶质瘤细胞迁移能力的改变.结果 敲除SENP1后的LN229细胞增殖与对照组相比明显被抑制,双染实验发现SENP1敲除可以促进肿瘤细胞凋亡.且进一步功能实验表明SENP1敲除后的肿瘤细胞的迁移能力明显下降.结论 SENP1可以促进细胞增殖,降低细胞凋亡率,并影响肿瘤细胞侵袭基因的表达,在神经胶质瘤发病和进展过程中发挥着十分重要的作用.