IL1B基因-31位点SNP与EV71手足口病的相关性研究

摘要

Objective To explore the association between IL-1B gene-31 single nucleotide polymorphisms(SNP)and susceptibility and severity of EV71 HFMD.Methods The genotype of IL-1B gene-31 SNP was determined in EV71 HFMD(n =1196,including 801 mild cases,395 severe cases)and healthy control group (HC,n =573)with TaqMan assay in a case-control study.(1)To explore the association between-31 SNP and susceptibility to EV71 HFMD;(2)To explore the association between-31 SNP and susceptibility to EV71 HFMD in different sex population;(3)To explored the association between-31 SNP and severity to EV71 HFMD.Results (1)IL-1B gene-31 T allele frequency was significantly higher in EV71 HFMD than that in HC.(2)IL-1B gene-31 T allele frequency was significantly higher in EV71 HFMD than in HC for male population.(3)The IL-1B gene-31 T is not associated with the severity of EV71 HFMD.Conclusion The IL-1B gene-31 T allele was associated with susceptibility to EV71 HFMD,but was not associated with the severity of EV71 HFMD,especially,the IL-1B gene-31 T allele was associated with susceptibility to EV71 HFMD for male population.%目的 探讨IL1B基因-31位点单核苷酸多态性(SNP)与EV71手足口病(HFMD)易感性及临床表型的相关性.方法 采用病例-对照研究方法,应用TaqMan探针基因分型技术,对1196例EV71 HFMD[(其中801例为轻症(M-EV71),395例为重症(S-EV71)]和573例健康对照人群(HC)IL1B基因-31位点进行分型,比较两组人群等位基因频率差异.(1)探讨IL1B基因-31位点SNP与EV71 HFMD易感性的相关性;(2)探讨在不同性别中-31位点SNP与EV71 HFMD易感性的相关性;(3)探讨-31位点SNP与EV71 HFMD临床表型的相关性.结果 (1) EV71 HFMD患者中IL1B基因-31 T等位基因的频率明显高于HC,这提示T等位基因增加了患EV71 HFMD的风险(OR=1.22;95％ CI,1.06 ～1.40;P =0.007);(2)男性EV71 HFMD患者中IL1B基因-31 T等位基因的频率明显高于男性HC,这提示T等位基因也增加了男性患EV71 HFMD的风险(OR=1.24;95％ CI,1.03 ～1.48;P =0.022);(3)IL1B基因-31位点SNP与EV71 HFMD的严重程度无相关性.结论 IL1B基因-31 T等位基因与EV71 HFMD易感性相关,而与EV71 HFMD严重程度无相关性;IL1B基因-31 T等位基因与男性EV71 HFMD的易感性也具有相关性.