血清乙型肝炎病毒包膜大蛋白检测与病毒复制的相关性研究

摘要

目的 通过检测慢性乙肝患者血清包膜大蛋白(HBV large envelope protein,HBV-LP)、乙肝病毒DNA(HBV DNA)和乙肝病毒e抗原(HBeAg),探讨HBV-LP对于反映体内乙肝病毒复制的意义.方法 对623例慢性乙肝患者血清标本采用酶联免疫吸附试验检测HBV-LP和HBeAg,实时荧光定量PCR检测HBV DNA.结果 慢性乙肝患者HBV-LP和HBV DNA阳性率明显高于HBeAg阳性率(x2=22.3和9.4,P＜0.01),HBV-LP和HBV DNA阳性率比较差异无统计学意义(x2=2.8,P＞o.05),HBeAg阳性或阴性患者血清HBV-LP和HBV DNA阳性率比较均差异无统计学意义(x2=3.2和0.6,P＞0.05).血清HBV-LP含量与HBV DNA拷贝数呈正相关(r=0.874,P＜0.01),在不同HBV DNA拷贝数组别间,HBV-LPA值差异有统计学意义(F =6.987,P＜0.01).100例健康对照者,其血清HBV DNA及HBV-LP测定结果均为阴性.结论 HBV-LP检测血清的灵敏度高于HBeAg,慢性乙肝患者血清HBV-LP与HBV DNA复制密切相关,可作为反映HBV复制的指标.%Objective To study the significance of serum hepatitis B virus large envelope protein (HBV-LP),HBV DNA and HBeAg in the diagnosis of viral replication.Methods In 623 chronic HBV patients,HBV-LP and HBeAg were detected by ELISA and HBV DNA was quantitaively detected by realtime polymerase chain reaction(PCR).Results The positive rate of HBV-LP and HBV DNA were higher than that of HBeAg in all chronic HBV patients(x2 =22.3 and 9.4,P ＜0.01),and the positive rate of HBVLP and HBV DNA was no significant difference (x2 =2.8,P ＞ 0.05),The positive rate of HBV-LP and HBV DNA detected in HBeAg(+) or HBeAg(-) were not significantly different(x2 =3.2 and 0.6,P ＞ 0.05).HBV DNA copies and A vaule of HBV-LP was a positive correlation(r =0.874,P ＜0.01),HBV DNA copies of different groups was significantly different from HBV-LP A values(F =6.987,P ＜0.01).In 100 healthy people,the detecting results of HBV DNA and HBV-LP are negetive.Conclusion HBV-LP was better than HBeAg for detection of HBV replication.There is a good correlation between the copies of HBV DNA and the levels of HBV-LP,HBV-LP expression can reflect the replication of HBV.