艾滋病病毒长期不进展者、缓慢进展者、高危暴露者以及正常人群APOBEC3G mRNA水平的差异

摘要

目的 检测我国中部地区艾滋病病毒(HIV)流行区4类不同人群APOBEC3G(hA3G)mRNA水平的差异并分析其与疾病进程的关系.方法 采集研究人群的静脉全血标本,分离外周血单核细胞(PBMCs),冻存于液氮中;复苏PBMCs,提取总RNA,mRNA体外逆转录为cDNA,应用实时定量聚合酶链反应(PCR)检测不同人群血标本APOBEC3G mRNA的水平.结果 HIV长期不进展者标本13份,其CD+4T淋巴细胞计数平均为(716±169)个/μl,平均感染时间为(12.5±2.3)年;HIV缓慢进展者标本48份,其CD4+T淋巴细胞计数平均为(233±144)个/μl,平均感染时间为(10.7±2.2)年.HIV长期不进展者APOBEC3G mRNA水平高于正常人群的水平;HIV缓慢进展者APOBEC3G mRNA水平高于高危人群和正常人群的水平.HIV长期不进展者和缓慢进展者的CD4+T淋巴细胞数量与APOBEC3G mRNA水平无相关性.结论 我国中部地区HIV流行区4类不同人群APOBEC3G mRNA水平存在差异,并且HIV长期不进展者和缓慢进展者CD+4T淋巴细胞数量与APOBEC3G mRNA水平无相关性.%Objective To examine the APOBEC3G(hA3G)mRNA levels of four different groups in the human immunodeficiency virus(HIV)prevalent areas in central China and to analyze the relationship between hA3G mRNA levels and HIV disease progression.Methods We collected peripheral blood and isolated the peripheral boold monouuclear cells(PBMCs),and then cryo-preserved the PBMCs in liquid nitrogen.Prior to the total extraction of RNA,PBMCs were resuscitated and mRNA were reverse Transcripted to cDNA in vitro.Real-time polymerase chain reaction(PCR)was used to test hA3G mRNA levels of different groups.Results There were 13 HIV long term non-progressors with the mean CD+4 T lymphocyte count as(716±169)per μl and the mean affection time as(12.5±2.3)years.There were 48HIV slow progressors with the mean CD+4 T lymphocyte count as(233±144)per μl and the mean affection time as(10.7±2.2)years.The hA3G mRNA level of HIV long term nonprogressors was higher than that of normal people while the hA3G mRNA level of HIV slow progressors was higher than that of normal people and high risk people.There were no correlations between CD+4 T lymphocyte count and hA3G mRNA levels of HIV long term nonprogressors as well as in HIV slow progressors.Conclusion There was difference found in the hA3G mRNA levels of four groups in the HIV popular area in central China while no correlation between CD+4 T lymphocyte count and hA3G mRNA levels of HIV long term nonprogressors as well as in HIV slow progressors were found.