Objective To investigate the combined effects of fluoride and aluminum intoxication on bones and their possible mechanisms.Methods Kunming mice were divided into nine groups according to the factorial experiment design.Different dose of fluoride(NaF,0,50,150 mg/L)and/or aluminum(AlCl3,0,200,600 mg/L)was administered to each group in drinking water.After 24 weeks,the degree of mottled teeth and the histomorphometric parameters,such as the bone trabecula and osteoid areas,the number of osteoblasts and osteoclasts,and pathologic changes in femur were observed.Results Aluminum could also caused mottled teeth(in degree 4).The mottled teeth in the combined groups were more serious than those in fluoride or aluminum alone group.The interaction between fluoride and aluminum existed in the changes of bone trabecula and osteoid areas(F=2.963,3.688,P＜0.05),and not existed in changes of the number of osteoblasts and osteoclasts(F=2.347,0.888,P＞0.05).In high fluoride group,the trabecula and osteoid areas were(50 675.47±22 916.34),(10 733.97 ±3015.55)μm2,but it increased to(75 988.64±13 797.21),(16 402.88±4605.83)μm2 when combined with high aluminum(P＜0.05),and the group of high fluoride +low aluminum increased to(69 277.16±19 837.51),(18 564.79±6362.47)μm2 (P＜0.05),so aluminum antagonized the effects induced by fluoride;the area of bone trabecula of group of high aluminum was(60 718.43 ±17 574.37)μm2,but it increased[(75 988.64±13 797.21),(82 474.94±15 466.66)μm2]when combined with high or low fluoride(P＜0.05),and the combined effects showed a similarity to those in high aluminum group.The prominent osteoporosis with increased osteoid and cartilage tissues,and decreased amount of bony matrix and minerals were the main histopathological changes in the bone.Conclusions Both high aluminum and fluoride intoxication can result in mottled teeth,their combined effects are more serious than the individual effect.The prominent injury of combined fluoride and aluminum intoxication is osteomalacia and osteoporosis.%目的 探讨氟铝联合中毒小鼠骨相组织病变特征及致病的可能机制.方法 采用析因设计方法,将昆明种小鼠分成9组,由氟化钠(NaF,0、50、150 mg/L)、氯化铝(AlCl3,0、200、600mg/L)经饮水染毒,24周后对斑釉牙发生情况进行分度,测量股骨下端骨骺的骨小梁面积、类骨质面积,计数成骨细胞和破骨细胞个数,并观察骨组织病理变化.结果 铝单独作用也可引起斑釉牙(分度为4度),氟铝联合对牙的损害比各自单独作用严重.氟铝共存对骨小梁面积和类骨质面积的影响存在交互作用(F值分别为2.963、3.688,P＜0.05),对成骨细胞和破骨细胞数的影响不存在交互作用(F值分别为2.347、0.888,P＞0.05).高氟组骨小梁面积、类骨质面积为(50675.47±22 916.34)、(10 733.97±3015.55)μm2,高氟高铝组升高到(75 988.64±13 797.21)、(16 402.88±4605.83)μm2(P＜0.05),高氟低铝组升高到(69 277.16 ±19 837.51)、(18 564.79 4-6362.47)μm2(P＜0.05),铝拮抗了氟的效应;高铝组骨小梁面积为(60 718.43 4-17 574.37)μm2,高氟高铝组[(75 988.64 ±13 797.21)μm2]、低氟高铝组[(82 474.94±15 466.6)μm2]均升高(P＜0.05),联合作用效应与高剂量铝的作用趋势一致.骨组织病理改变为骨质疏松明显,类骨质和软骨组织增多,骨基质和矿化骨减少.结论 高剂量的铝和氟均可引起斑釉牙,联合作用比单一因素作用强.氟铝联合中毒对骨骼的影响以软化型骨质疏松为主要特征.
展开▼
