鼠疫F1抗体三种血清学检测方法比较

摘要

Objective To compare the effect of three serological methods for detection of Yersina pestis F1 antibody.Methods F1 antibody of Yersinapestis was detected with the methods of enzyme linked immunosorbent assay(EL1SA),indirect hemagglutination assay(IHA) and gold-immunochromatography assay (GICA),respectively.Results The highest antibody titer was 1 ∶ 5120 by ELISA and 1 ∶ 640 by IHA.Meanwhile,the highest antibody titer of GICA was 1∶ 1280.Conclusions EL1SA is the most sensitive method in detection of Yersina pestis F1 antibody.The sensitivity of GICA is low and that of IHA is the lowest of three serological methods.%目的 比较鼠疫F1抗体三种血清学检测方法的效果.方法 通过酶联免疫吸附试验(ELISA)、试管法间接血凝试验(IHA)和胶体金纸上色谱试验(GICA)三种血清学检测方法检测血清中鼠疫F1抗体.结果 ELISA检测F1抗体的最高抗体滴度为1∶5120,IHA的最高抗体滴度为1∶640,GICA的最高抗体滴度为1∶1280.结论 三种血清学检测方法中酶联免疫吸附试验(ELISA)敏感性最高,胶体金纸上色谱(GICA)次之,试管法间接血凝试验(IHA)最低.