三种方法检测鼠疫抗体在准噶尔盆地鼠疫监测中的应用与分析

摘要

目的 比较鼠疫监测中酶联免疫吸附试验夹心法(ELISA)与间接血凝试验(IHA)、胶体金试验(GICA)检测鼠疫F1抗体的应用效果.方法 三种方法同步检测,比较鼠疫F1抗体的阳性检出率、反应滴度.结果 在9个地区,采集血清265份;ELISA、IHA、GICA三种方法检出鼠疫F1抗体的阳性率分别为10.56％(28/265)、7.17％(19/265)、6.79％(18/265);ELISA的平均滴度(25.107)高于IHA (23.894,t=2.358,P＜0.05).结论 ELISA的敏感性高于IHA、GICA;为选定适用的检测鼠疫F1抗体方法提供了参考资料.两种或两种方法以上方法同步用于鼠疫监测,可避免或减少重要数据错误造成的损失.%Objective To compare the effects of enzyme linked immunosorbent assay(ELISA),indirect hemagglutination test(IHA) and colloidal gold immunochromatography (GICA) in detecting plague F1 antibody in Junggar Basin.Methods The three methods were applied synchronously,and the positive rates and reaction titers of plague F1 antibody were compared.Results Of the 265 samples of rodent serum collected in the nine regions,the positive rates of ELISA,IHA and GICA were 10.5％ (28/265),7.17％ (19/265) and 6.79％ (18/265),respectively; the average titers of ELISA(25.107) was higher than that of IHA(23.894,t =2.358,P ＜ 0.05).Conclusions The sensitivity of ELISA is higher than that of IHA and GICA,which provides a useful information in choosing appropriate method for detection of plague F1 antibody.Synchronized application of the three methods in plague surveillance could improve the accuracy of surveillance data.