亚慢性染氟对雌性小鼠雌激素受体mRNA表达的影响

摘要

Objective To study the subchronic toxicity of sodium fluoride on mRNA expression of estrogen receptor(ER) in female mice.Methods Forty female mice were randomly divided into 4 groups according to body mass,10 in each group,and exposed to sodium fluoride solution(0,1,5,25 mg/L) through drinking water in control,low-dose,medium-dose and high-dose groups for 12 weeks.The expression levels of ERα,ER3 mRNA in ovarian tissues were determined by RT-PCR method.Results The relative expression levels of ERα,ERβ mRNA in control,low-,medium-and high-dose groups were 0.7028 + 0.0474,0.7195 ± 0.0552,0.6479 ± 0.0590,0.5684 ± 0.0513 and 0.8418 ± 0.0131,0.7729 ± 0.0974,0.7610 ± 0.0984,0.8026 ± 0.0234,respectively.The difference between high-dose and control groups of the expression level of ERα was statistically significant (P ＜ 0.05).Conclusions The subchronic toxicity of fluoride can decrease the expression of estrogen receptor in ovarian tissue,which may have a certain effect on reproductive development of female mice.%目的 观察亚慢性染氟(氟化钠)对雌性小鼠雌激素受体(ER)mRNA表达的影响.方法 雌性小鼠40只,按体质量随机分成4组:对照组、低氟组、中氟组、高氟组,每组10只.分别饮用0、1、5、25 mg/L氟化钠溶液,喂养12周后,RT-PCR法检测小鼠卵巢中ERα、ERβ mRNA表达.结果 对照组、低氟组、中氟组、高氟组小鼠卵巢组织ERα、ERβ mRNA表达分别为0.7028±0.0474、0.7195±0.0552、0.6479±0.0590、0.5684±0.0513,0.8418±0.0131、0.7729±0.0974、0.7610±0.0984、0.8026±0.0234.其中高氟组小鼠卵巢组织ERαmRNA表达与对照组比较,差异有统计学意义(P＜0.05).结论 亚慢性染氟可导致卵巢组织中雌激素受体表达下降,后者对雌性小鼠的生殖发育可能有一定的影响作用.