可溶性CD44v6、VEGF检测在结核性腹水和卵巢癌性腹水鉴别诊断中的价值

摘要

Objective To investigate the diagnostic value of soluble CD44v6 ( sCD44v6 ) and VEGF in the tuberculous ascites and ovarian cancer ascites.Methods Used enzyme linked immunosorbent assay ( ELISA ) to detect sCD44v6 and VEGF levels in 39 cases of ovarian cancer ascites, and 48 cases of tuberculous ascites.Results sCD44v6 was ( 102 ± 31 ) ng/ml in ovarian cancer ascites group, which was significantly higher than tuberculous ascites( 44 ± 15 ) ng/ml( P ＜ 0.01 ).VEGF was( 684 ± 210 ) ng/ml in ovarian cancer ascites, which was significant different from tuberculous ascites( 90 ± 11 )ng /ml( P ＜ 0.01 ).sCD44v6 ＞ 60.5 ng/ml was defined as positive critical values, diagnosis of ovarian cancer ascites by sCD44v6 were as followed, sensitivity was 82.1％ ( 32/39 ), specificity was 87.5％ ( 42/48 ); VEGF ＞ 101.3 ng/ml were defined as positive critical values, diagnosis of ovarian ascites by VEGF were as followed, sensitivity was 92.3 ％ ( 36/39 ),specificity was 89.9％ ( 43/48 ).Conclusion CD44v6 and VEGF in malignant ascites were significantly increased, detection of sCD44v6 and VEGF were of great value in differentiate ascites of tuberculosis and ovarian cancer.%目的 探讨可溶性CD44变异性外量子(sCD44v6)及血管内皮生长因子(VEGF)对结核性、卵巢癌性腹水的诊断价值.方法 选取39例卵巢癌性腹水、48例结核性腹水采用酶联免疫吸附试验(ELISA)检测腹水中sCD44v6及VEGF的水平,并进行相关性分析.结果 卵巢癌性腹水组sCD44v6水平为(102±31) ng/ml,明显高于结核性腹水组(44±15)ng/ ml,差异有统计学意义(P<0.01).卵巢癌性腹水组VEGF水平为(684 ±210) ng/ml,明显高于结核性腹水组(90 ±11)ng/ml,差异有统计学意义(P<0.01).sCD44v6以>60.5 ng/ml为阳性界值,sCD44v6诊断卵巢癌性腹水的敏感性为82.1% (32/39),特异性为87.5%(42/48);VEGF以>101.3 ng/ml为阳性界值,VEGF诊断卵巢癌性腹水的敏感性为92.3%(36/39),特异性为89.9%(43/48).结论 癌性腹水中的CD44v6、VEGF水平明显升高,sCD44v6、VEGF的检测对于结核性、卵巢癌性腹水的鉴别诊断有重要价值.