IL-4对NOD小鼠1型糖尿病免疫干预及其机制的探讨

摘要

Objective　To study the effectiveness of IL-4 o n type 1 diabetes in NOD mice and it's possible mechanism.Methods 　4-week-old NOD mice,as a kind of type 1 diabetes model,were treat ed with IL-4 to investigate the incidence of insulitis,and lymphocyte subsets were determined by FACS.Results　The incidence of diabetes in the IL-4 group was 12.50% which was obviously less than 62.50% in the contr ol group (P＜0.05),and the degree of insulitis in the IL-4 group was also l ess than that in the control group.The lymphocyte subset in the IL-4 treated gr oup showed higher CD+4/CD+8 cells and percentage of IL-2R+ cells in s pleen,and higher proportion of CD+4/CD+8 but decreased CD+4/CD+8 cells in thymus.Conclusion　IL-4 can prevent NOD mice fr om developing type 1 diabetes,which may be related to the fact that IL-4 c orrected the imbalance of immunoregulatory in NOD mice.%目的探讨IL-4对NOD鼠1型糖尿病发病率、胰岛炎的影响及其机制。方法采用人类1型糖尿病动物模型NOD(nonobe se diabetic)鼠，IL-4处理后检测血糖、尿糖及糖尿病发病率，HE染色观察胰岛炎程度，流式细胞仪测定周围及中枢淋巴细胞亚群的变化。结果 IL-4处理组糖尿病发病率为12.50%，明显低于对照组(62.50%)(P＜0.05)，且胰岛炎的严重程度亦低于对照组；处理组周围单个核细胞CD+4/CD+8及IL-2R+细胞百分比增高，胸腺CD+4 CD+8细胞百分比增高而CD+4 CD+8细胞百分比下降(P＜0.01) 。结论 IL-4有降低NOD鼠1型糖尿病发病率及胰岛炎严重程度的作用；这种作用可能与IL-4影响了淋巴细胞亚群的分布从而纠正了免疫调节失衡有关。