糖耐量正常的2型糖尿病一级亲属氧化应激水平与胰岛素敏感性的相关性研究

摘要

目的 观察2型糖尿病(T2DM)患者及糖耐量正常的T2DM一级亲属(FDRs)体内氧化应激状态,探讨氧化应激与胰岛素敏感性之间的关系.方法 检测外周血清丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、8-异前列腺素F2α(8-iso-PGF2α)水平,高胰岛素正葡萄糖钳夹技术评估胰岛素敏感性.结果 T2DM组、FDRs组较NC组MDA、8-iso-PGF2α水平升高(P＜0.05),而血GSH-PX、SOD水平降低,差异有统计学意义(P＜0.05);MDA、8-is0-PGF2α与葡萄糖输注速率(GIR)呈明显负相关(r=-0.415和-0.443,P＜0.05),GSH-PX、SOD与GIR呈明显正相关(r=0.327和0.439,P＜0.05).结论 T2DM患者及糖耐量正常的T2DM一级亲属存在氧化应激状态的改变,且与胰岛素敏感性密切相关.%Objective To study the relationship of serum oxidative stress with insulin sensitivity in type 2 diabetes mellitus (T2DM) and NGT first degree relatives (FDRs) of T2DM.Methods Fasting level of MDA, GSH-PX, SOD, 8-iso-PG-F2α, and FFA was measured in T2DM patients, NGT first degree relatives (FDRs) of T2DM and normal controls.Insulin sensitivity was evaluated by euglycemic hyperinsulinemia clamp technique.Results The level of MDA and 8-iso-PG-F2α was significantly higher in both T2DM and FDRs than that in control group (P＜0.05), and the level of GSH-PX and SOD was lower in FDRs than in controls and higher in FDRs than in diabetics.Conclusions Oxidative stress may play an important role in the development of T2DM.