黄芩素、氨基胍干预下糖尿病大鼠类表皮样生长因子结构蛋白-7脑组织表达变化的研究

摘要

目的 探讨黄芩素、氨基胍干预对糖尿病大鼠脑组织中人类表皮样生长因子结构蛋白-7(EGFL-7)表达的影响.方法 选取6~8周龄健康雄性SD大鼠40只,随机分为正常对照(NC)组、糖尿病组、氨基胍组、黄芩素组,每组各10只.NC组予普通饮食喂养;糖尿病组、氨基胍组、黄芩素组建立糖尿病大鼠模型,氨基胍组、黄芩素组分别予氨基胍、黄芩素溶液灌胃.检测各组脑组织EGFL-7表达情况,观察各组脑血管内皮超微结构.结果 与NC组比较,糖尿病组FPG升高,胰岛素降低(P<0.05);与糖尿病组比较,氨基胍组和黄芩素组FPG降低,胰岛素升高(P<0.05).NC组毛细血管腔规则,糖尿病组毛细血管腔不规则,部分狭窄变形,血管周围可见大量水肿液,氨基胍组部分血管形态不规则,黄芩素组血管内皮细胞结构清晰;与NC组比较,糖尿病组EGFL-7表达阳性细胞数、相对灰度值升高[(0.41±0.18)%vs(19.20±2.56)%,(25.32±3.01)%vs(60.10±5.73)%,P<0.05];与糖尿病组比较,氨基胍组和黄芩素组EGFL-7表达阳性细胞数、相对灰度值降低[(19.20±2.56)%vs(14.83±2.31)%、(13.89±3.08)%,(60.10±5.73)%vs(35.90±4.91)%、(34.12±5.04)%,P<0.05].结论 黄芩素、氨基胍可影响糖尿病大鼠脑血管增生和重塑,其作用可能与降低脑组织EGFL-7表达有关.%Objective To investigate the effect of Baicalein and aminoguanidineon on the expression of human epidermal growth factor like domain protein -7 (EGFL-7) in brain tissue of diabetic rats. Methods A total of 40 male SD rats were selected in this study. They were 6 to 8-week-old and randomly divided into four groups :normal control group (NC group ,n = 10 ) ,diabetes group ( n = 10 ) , aminoguanidine group (n=10) ,and Baicalein group (n= 10). NC group was treated with normal diet. Diabetic rat models were established in diabetes group ,aminoguanidine group and Baicalein group. Aminoguanidine group and Baicaleingroup were treated with aminoguanidine and Baicalein solution gavage respectively. The expressions of EGFL-7 in brain tissues were detected and the ultrastructure of cerebral vascular endothelial was observed. Results FPG increased and insulin decreased in diabetes group than in NC group (P<0.05). FPG decreased and insulin increased in aminoguanidine group and Baicalein group than in diabetes group(P<0.05). The capillary lumen was normal in NC group ,but irregular in diabetes group ,showing narrow deformation and a large amount of edema fluid in perivascular area. Part of the capillary lumen was irregular in aminoguanidine group. Vascular endothelial cell structure was clear in Baicalein group. The expression of EGFL-7 positive cell number [(19.20 ± 2.56)% vs (0.41 ± 0.18)% ] , and relative gray value [(60.10 ± 5.73 )% vs (25.32 ± 3.01 )% ] were significantly higher in diabetes group than in NC group (P<0.05). The expression of EGFL-7 positive cell number [(14.83 ± 2.31)% , (13.89 ± 3.08)% vs (19.20 ± 2.56)% ] and relative gray value [(35.90 ± 4.91)% ,(34.12 ± 5.04)% vs (60.10 ± 5.73)% ] were significantly lower in aminoguanidine group and Baicalein group than in diabetes group (P<0.05). Conclusion Baicalein and aminoguanidine can affect the cerebral vascular proliferation and remodeling in diabetic rats ,w hich may due to reduced expression of EGFL-7 in brain tissue.