心肌组织特异性microRNA-1在ST段抬高性急性心肌梗死患者血中含量变化的研究

摘要

目的 通过检测心肌组织特异性microRNA-1(miR-1)在ST段抬高性急性心肌梗死(acute myocardial infarction,AMI)患者血浆中的含量,探讨血浆miR-1在AMI诊断中的作用.方法 20例AMI患者在发病12 h内取血,20例健康志愿者血作为对照,用TaqMan实时荧光定量RT-PCR(TaqMan real-time quantity RT-PCR,qRT-PCR)检测血中miR-1含量,并同时检测血清肌钙蛋白I(cTnI)和肌酸激酶同工酶(CK-MB).结果 AMI患者血清cTnI、CK-MB和血浆miR-1含量明显高于正常值(P<0.01),AMI患者血浆miR-1含量与血清CK-MB和cTnI含量呈直线正相关(P<0.05).结论 血浆miR-1可作为AMI的一个新的敏感生物学标志.%Objective To observe serum cardio - specific microRNA -1 (miR-1) levels in ST segment elevation acute myocardial infarction patients, and to explore the effect of serum miR - 1 levels on the diagnosis of acute myocardial infarction ( AMI). Methods We assessed the serum concentrations of miR - 1 in 20 patients with AMI, and 20 healthy individuals as control. Serum miR -1 concentrations were measured with a real - time reverse - transcription PCR ( qRT - PCR) , serum cardiac troponin I (cTnl) or creatine kinase - MB( CK - MB) concentrations were measured. Results Serum level of cTnl, CK - MB and miR - 1 were obviously higher in AMI patients than in healthy individuals (P<0.01) ; The serum miR - 1 level in the patients with AMI had a positive correlation with serum cTnl or CK - MB ( P < 0. 05 ). Conclusion Serum miR - 1 could be a novel sensitive biomarker of AMI.