脓毒症肝损伤中凝血酶敏感蛋白-1的变化及意义

摘要

目的 探讨凝血酶敏感蛋白-1(TSP-1)在脓毒症肝损伤中的变化及意义.方法 雄性Balb/c小鼠通过盲肠结扎穿刺(CLP)成功制作脓毒症模型,分为对照组,CLP后0.5、1、3、6、12、24 h七个组(每组5只),观察腹腔及肝脏大体解剖,检测血清转氨酶含量、肝脏TSP-1 mRNA及TSP-1蛋白表达水平,从而探讨脓毒症时肝脏TSP-1的变化规律.结果 与对照组相比,制作脓毒症模型后6 h肝脏TSP-1 mRNA及TSP-1蛋白表达明显增高,同时伴有肝脏炎症加重、血清ALT和AST含量明显升高,在制模24 h观察期内TSP-1维持于较高水平.结论 脓毒症时肝脏TSP-1表达增加,TSP-1作为炎症介质可能参与介导脓毒症肝损伤发生、发展.%Objective To explore ihe alleralion and significance of ihe ihrombospondin - 1 in seplic liver injury. Methods The model for sepsis was developed by cecal ligalion and punclure ( CLP) in male Balb/c mice. All mice were divided inlo seven groups; control, O.5h,lh,3h,6h, 12h,24 h afler ihe CLP model for sepsis(n =5). Then ihe general analomical fealure of abdominal cavily and liver was observed. Serum aminolransferase, expression level of liver TSP - 1 mRNA and TSP - 1 prolein were delecled. The pallern of liver TSP - 1 in sepsis was investigated. Results In comparison with control group, the expression of liver TSP - 1 mRNA and TSP - 1 protein was significantly increased accompanied with more severe liver inflammation as well as dramatically increasing serum ALT and AST contents 6 h after the CLP model for sepsis. TSP - 1 maintained a relatively high level during 24 h observation period. Conclusion In sepsis the expression of liver TSP - 1 is increased which may induce and develop septic liver injury as a mediator of inflammation.