气管切开插管留置后大鼠外周血β-防御素-2及IL-2的改变

摘要

Objective To explore the beta defense-2(BD-2)mRNA expression and interleu⁃kin-2(IL-2)content in the peripheral blood of rats after tracheotomy. Methods All of 48 SPF health male SD rats were divided into two groups: normal control group(A)and model with tracheotomy tube (B). After successfully setting up the tracheotomy model, we collected the specimen 24 h, 72 h, and 168 h after drug administration. Peripheral blood was collected for the detection of BD- 2 mRNA expression and content of IL-2. Results ①The BD2 mRNA expression quantity decreased obviously in peripher⁃al blood of rats after tracheotomy, especially at 24 h, the degree of decline slightly improved at the 72 h and 168 h. ②The IL-2 content did not change in peripheral blood of rats after tracheotomy. Conclu-sion The BD2 mRNA expression in the peripheral blood of rats is significantly increased after tracheot⁃omy, and then it is compensatory increased, and subsequently continued to decline. It may suggest that the immune function of rats is low after tracheotomy.%目的：探讨气管切开插管留置后大鼠外周血中β-防御素-2（BD2）mRNA表达、白细胞介素-2（IL-2）的变化规律。方法将48只SPF级健康雄性Sprague-Dawley（SD）大鼠分成正常对照组（A）、气管切开插管留置模型非用药组（B），每组各24只。制备大鼠气管切开插管留置模型。模型成功后，在给药后第24、72、168小时三个时段活体取材。采集外周血标本，检测血清BD2 mRNA 表达和 IL-2含量。结果①气切插管后大鼠外周血 BD2表达量明显下降，以气管切开后第24小时最明显，在第72小时、168小时两个时间段下降程度稍有改善。②气管切开后大鼠外周血IL-2含量没有改变。结论气管切开插管后大鼠外周血BD-2 mRNA的表达量先下降，后代偿性升高，后期持续下降；提示气管切开插管留置后大鼠可能存在免疫功能低下。