淫羊藿对气管切开插管留置大鼠免疫功能的影响

摘要

目的 探讨淫羊藿对气管切开插管留置大鼠免疫功能的影响.方法 将54只SD大鼠随机分为正常对照组(A组)、模型非用药组(B组)、模型用药组(C组),每组18只.B组及C组建立气管切开插管留置大鼠模型.造模6 h后C组给予灌胃淫羊藿水提取物,其余两组灌食等量生理盐水.分别于灌胃后第24小时、第72小时、第168小时,每组取6只大鼠测定肺组织β-防御素-2(BD-2)mRNA表达水平、外周血白细胞介素(IL)-2及T细胞亚群的水平.结果 (1)B组第72小时、168小时的BD-2 mRNA相对表达量均低于第24小时(P<0.05),与A组比较,B组第24小时的BD-2 mRNA相对表达量升高但第168小时时却降低(P<0.05);第24小时、72小时及168小时,C组的BD-2 mRNA相对表达量均高于B组(P<0.05).(2)C组第72、168小时的IL-2及CD4 +水平均高于B组(均P<0.05);在第24小时,B组的CD3 +水平低于A组,但C组高于B组(均P<0.05);3组第24、72小时的CD8 +水平比较,差异均无统计学意义(P>0.05),但至第168小时, C组的CD8 +水平低于A组(P<0.05).结论 淫羊藿可能通过上调肺组织BD-2 mRNA及血CD4 +T细胞、IL-2的表达,下调CD8 +T细胞的表达,从而改善气管切开插管留置大鼠免疫功能.%Objective To explore the effect of Epimedium brevicornu Maxim. on immune function in rats with trachea cannula retention after tracheostomy.Methods Fifty-four SD rats were randomly divided into normal control group(group A),model group without medication(group B),and model group with medication(group C),with 18 rats in each group.Rat model with tracheostomy and trachea cannula retention was established in group B and group C.After 6 h of modeling,intragastric administration with water extract of Epimedium brevicornu Maxim.was performed in group C,and intragastric administration with same volume of normal saline was performed in the other two groups.At the 24th,72th and 168th hour after intragastric administration,β-defensin-2(BD-2) mRNA expression level in the lung tissues,interleukin 2(IL-2) level and T cell subsets counts in the peripheral blood were detected in 6 rats of each group.Results (1)In group B,the relative expression levels of BD-2 mRNA at the 72th and 168th hour were lower than the level at the 24th hour(P<0.05), and the level in group B increased at the 24th hour but decreased at the 168th hour compared to the level in group A(P<0.05).At the 24th,72th and 168th hour,the relative expression levels of BD-2 mRNA were higher in group C compared to the levels in group B(P<0.05). (2)At the 72th and 168th hour,the levels of IL-2 and CD4+were higher in group C compared to the levels in group B(all P<0.05).At the 24th hour,the CD3+level in group B was lower than that in group A,but the level in group C was higher than that in group B(all P<0.05). There were no significant differences in the CD8+level at the 24th or 72th hour among the three groups(P>0.05),but the level at the 168th hour was lower in group C compared to the level in group A(P <0.05).Conclusion Epimedium brevicornu Maxim. can improve the immune function in rats with trachea cannula retention after tracheostomy by up-regulating the expression of BD-2 mRNA in pulmonary tissues and the expressions of CD4+T cell and IL-2 in blood as well as down-regulating the expression of CD4+T cell.