五倍子单宁酸影响变异链球菌生物膜形成的体外研究

摘要

AIM: To observe the effects of gallnut tannic acid on Streptococcus mutans plaque biofilm forma-rntion in Bioflux System. METHODS: Streptococcus mutans was incubated in the observation zone of Bioflux200 System for 30 min, then BHIS solution (control group) , BHIS containing 8mg/mL or 16mg/mL Gallnut tannic acid, BHIS solution containing 2 g/L chlorhexidine (positive control group) were added to the inlet respectively. The bacteria were continuously cultured under 0. 8 dyne shear force for 12 h. The biofilm formation was observed under confocal laser scanning microscope after staining with LIVE/DEAD ? BacLightTM Bacterial Viability Kit. RESULTS; Complete plaque biofilm was formed in the control group, and most bacteria was live. No biofilm formation was observed in the 2 gallnut tanic acid treatment groups, only a few of sparsely distributed live and dead bacteria could be found. In the chlorhexidine group, no plaque biofilm formation was observed and only scattered dead bacteria could be seen. CONCLUSION ; Gallnut tannic acid can inhibit the Streptococcus mutans biofilm formation under shear force.%目的:在微流体系统(Bioflux System)中观察五倍子单宁酸对变异链球菌生物膜形成的影响作用.方法:Bioflux200系统中接种复苏的变异链球菌至观察区,静置30 min后,分别在各进孔加入BHIS液(对照组)、含五倍子单宁酸终末浓度分为8 mg/mL(实验1组)、16 mg/mL(实验2组)的BHIS液及含2 g/L洗必泰的BHIS液(阳性对照组)；然后在0.8 dyne剪切力作用下连续培养12h；活菌死菌荧光染色剂(LIVE/DEAD(R)BacLightTM)染色后在激光共聚焦扫描显微镜下观察各组生物膜的形成情况.结果:对照组形成了较完整的菌斑生物膜且视野内多为活菌；两种浓度五倍子单宁酸组均无菌斑生物膜形成,只可观察到少量散在的死菌和活菌；洗必泰组亦无菌斑生物膜的形成,且视野内基本为散在的死菌存在.结论:一定浓度五倍子单宁酸在剪切力的作用下可抑制变异链球菌菌斑生物膜的形成.