AIM:To evaluate the effect of Astragalus polysaccharides ( APS) on the proliferation, differentia-tion and mineralization of MC3T3-E1 cells in high glucose conditions and to study the underlying mechanisms. METH-ODS:MC3T3-E1 cells were cultured in high-glucose medium containing 0. 05(LDA), 0. 5(MDA) and 5(HDA) mg/mL of APS respectively. Cell proliferation, differentiation and mineralization were examined by MTT assay, ALP Kit and Alizarin red dye staining respectively. The expression of Runx-2, OPN and OCN were examined by RT-PCR. The cytoskeleton of the cells was observed by confocal laser scanning microscopy ( CLSM) . RESULTS: APS promoted the proliferation of MC3T3-E1 cells by LDA and MDA(P<0. 05). LDA and MDA significantly increased the ALP activity and calcified nodules (P<0. 05). Furthermore, LDA increased the gene expressions of Runx-2, OPN and OCN in a time-dependent manner (P<0. 05). CLSM observation showed that the cells in LDA group were more regular in struc-ture and had more extensions than those in the other groups at each time point. CONCLUSION: Under high glucose conditions, 0. 05 mg/mL APS promotes the proliferation, differentiation and mineralization of MC3T3-E1 cells. The effect is related to its promotion of the expression of OPN, Runx-2 and OCN.%目的：：探讨高糖环境下黄芪多糖(伯恩)对细胞MC3T3-E1增殖、分化和矿化的影响。方法：将MC3T3-E1分别与含黄芪多糖浓度为5、0.5、0.05 mg/mL的高糖培养基(分别为HDA组、MDA组、LDA组)和单纯高糖培养基(对照组)共同培养后,分别通过MTT法、碱性磷酸酶和茜素红染色法观察各组MC3T3-E1细胞的增殖、分化和矿化活性；激光共聚焦显微镜观察各组细胞的超微骨架结构；实时荧光定量PCR检测各组细胞中骨活性相关基因 Runx-2、OPN、OCNmRNA 的表达水平。结果：在高糖环境下 LDA、MDA 组更利于MC3T3-E1细胞的增殖和分化,与HDA组和对照组相比P<0.05； LDA组MC3T3-E1细胞的矿化程度及细胞超微骨架结构均明显优于其他各组(P<0.05)； RT-PCR检测显示,LDA组能显著增加 MC3T3-E1细胞中OPN、Runx-2、OCN mRNA的表达水平(P<0.05)。结论：高糖环境下,LDA组有利于MC3T3-E1细胞的增殖、分化和矿化；其对细胞活性的影响,与促进细胞骨架的排列、伸展和增加Runx-2、OPN、OCN的表达量有关。
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