一种改进的非人灵长类神经功能缺损评价方法

摘要

目的 对文献中的量表进行修改和细化后用于实验猴的神经功能动态量化观察,使其更适于灵长类脑卒中神经功能缺损的评价.方法 成年食蟹猴12只,用自体股动脉抗凝血脑内立体注射的方法建立脑出血模型,造模后1周将动物随机分为对照组、干细胞治疗高剂量组和低剂量组,分别在出血灶周围注射生理盐水、脊髓间充质干细胞5×10(6)/只和1×10(6)/只,造模6h,1d,3d,lw后及干细胞注射后1d,3d,1w,2w,3w,4W在每天的固定时间使用文献中的表格和/或改进的表格进行评分,比较造模前后和干细胞注射前后动物受损伤侧上肢神经功能缺损情况评价造模和干细胞治疗效果.结果 造模手术前,所有动物的行为表现均正常,神经功能评分为0.造模手术后6h,用修改前后的表格评分均为31分左右.但从术后3d开始出现评分上的差异.用修改后的表格对动物的神经功能进行评分结果 显示干细胞注射后1周开始到动物被安乐时,剂量组与对照组之间的神经功能评分出现显著性差异(P＜0.05),离、低剂量组间未见显著性差异.结论 改进的表格克服了旧表格中分差大,评价标准模糊的缺点,提高了量表评价的敏感性,具有良好的临床相似性和可操作性.为建立标准化神经功能评价量表进行了有益尝试.%Objective Use the proved Non-human Primate Neurological deficit scale to evaluate the effect of Intracerebral Hemorrhage model of Cynomolgus macaques Treatment with Human Bone Marrow Mesenehymal Stem Cells. Methods 12 adult male Cynomolgus monkeys were used in this study. The Intracerebral Hemorrhage model were made by using the autologous anticoagulated femoral artery blood injection. 12 monkeys were divided into three groups one week after the surgery. The control group consisted of four animals receiving physiological saline in the volume of 250 u,l 3 mm away from the outside edge of the infarcts. The high-dose and low-dose group receiving Human Mesenehymal Stem Cell(hMSC) with the cell number of 5 x 106 and 1 x 106 at the same place and volume with the control group. Use the Non-human Primate Neurological deficit scale and/or proved to evaluate the effect of the stem cell at the 6h,ld,3d,lweek after surgery and 1,3 day and 1,2,3,4 weeks after transplantation. Result Compared with the group, the score of the dose-group animals much slower than that in the control group after transplantation(p < 0. OS). There is no significant difference between high-dose and low-dose group. Conclusion The proved Non-human Primate neurological deficit scale is more objectively and accurately than the original one and can be used to measure neuronal loss in vivo and to evaluate the effects of therapeutic strategies involving neural or stem cell transplantation.