PMSG或FSH同期发情处理对小鼠卵巢、输卵管和子宫中孕激素受体分布的影响

摘要

Objective To investigate the distribution of Progesterone Receptor(PR) in murine ovary, fallopian tube and uterus between the mice treated by estrus synchronization of PMSG (pregnant mare serum gonadotropin ) and of FSH (follicle stimulating hormone). Methods Total 10 KM female mice were divided into two groups: PMSG and FSH treatment group, after the injection of these hormones for 48 h, all of their ovaries, oviducts and uterus were fixed by paraform, and stained by immunohistochemical staining to observe and analyze the distribution of PR in these tissues.Results The positively stained cell number in the primary and secondary ovarian follicles of the PMSG treatment group,were significantly higher than those of the FSH one ( P ＜ 0. 05 ), the average absorbance in the ovarian follicles of the PMSG treatment group was significantly higher than that of the FSH one ( P ＜ 0. 05 ). In addition, The positively stained cell number in oviduct of the PMSG treatment group was significantly higher than those of the FSH group( P ＜ 0. 05 ) ,but the average absorbance is reversed. In endometrial epithelium and glandular epithelium, the positively stained cell number of the PMSG treatment group was significantly higher than that in the FSH one ( P ＜ 0. 05 ), the average absorbance in endometrial stroma and endometrium of the PMSG treatment group was significantly lower than that of the FSH one ( P ＜0. 05 ). Conclusions PMSG and FSH may induce varying degrees of impact on the distribution of PR in murine ovaries,oviducts and uterus, the expression of PR of the PMSG treatment group was significantly higher than that of the FSH one.%目的 探讨孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)和促卵泡激素(follicle stimulating hormone,FSH)同期发情处理的小鼠卵巢、输卵管和子宫中孕激素受体(progesterone receptor,PR)分布的差异.方法 10只8周龄KM系雌鼠,随机分为PMSG和FSH两个组,第一次处理后48 h取其卵巢、输卵管、子宫进行固定,免疫组织化学法观察各组织中PR的分布.结果 两组小鼠卵巢、输卵管和子宫内膜中均有PR表达;其中PMSG组初级卵泡、次级卵泡和输卵管的阳性率均显著高于FSH处理组(P＜0.05);PMSG组各级卵泡的平均吸光度值均显著高于FSH处理组(P＜0.05);PMSG处理组子宫内膜上皮与腺上皮中的阳性率显著高于FSH组(P＜0.05).而基质和子宫内膜上皮中的平均吸光度值显著低于FSH组(P＜0.05).结论 PMSG和FSH同期发情处理不同程度影响小鼠卵巢、输卵管和子宫中PR的表达分布;其中PMSG处理组小鼠卵巢、输卵管和子宫上皮中PR表达普遍显著高于FSH处理组.