愤怒情绪模型大鼠不同脑区γ氨基丁酸B2受体表达的变化

摘要

目的 通过栓测愤怒情绪模型大鼠顶区皮质和海马γ氨基丁酸B2受体(GABA,112)表达的变化,探索愤怒情绪与GABABR2的关系,以及中药经前平颗粒的干预机制.方法 采用居住人侵的方法复制愤怒情绪模型大鼠,然后用RT-PCR和Western Blot的方法检测GABAB R2 mRNA和蛋白的表达差异.结果 与正常组相比,模型组大鼠GABABR2 mRNA和蛋白的表达都明显降低;与模型组相比,经前平给药组大鼠GABABR2和蛋白的表达都显著升高.结论 大鼠顶区皮质和海马GABABR2表达降低可能是影响大鼠绪怒情绪的重要机制之一;中药经前平颗粒可对GABABR2表达异常变化起到调节作用.%Objective To research the relationship of anger model and γ-aminobutyric acid B2 receptor ( GABAB R2), and the intervention mechanism of jingqianping granule by observing the differential expression of GABABR2 in anger emotional model rats of cerebral cortex regions and hippocampus. Methods The anger model was induced in rats by resident-intruder. We analyzed the expression of GABABR2 in cerebral cortex and hippocampus by Western Blot and RT-PCR. Results The expression of GABABR2 in cerebral cortex and hippocampus of model rats decreased obviously compared with the normal control group. Compared with the model group, the expression of GABABR2 increased after treated with Jingqianping graulne. Conclusion The Jingqianping granule can up-regulate the GABAsR2 expression level of the model rats in cerebral cortex and hippocampus, which may be one of the mechanisms impacting rats with anger emotion. The expression changes of GABABR2 are tight related to rats with anger emotion.