猴免疫缺陷病毒（SIVmac239）多次直肠暴露对机体细胞免疫的影响

摘要

Objective To study the effect of repeated rectal exposure of low -dose simian immunodeficiency virus on the systemic cellular immunity in monkeys .Methods Eight 3-to 4-year old rhesus macaques ( Macaca mulatta) (male:female 1:1) were used in this study.The monkeys were inoculated with 10 TCID50 SIVmac239 virus through rectum twice a week for consecutive 6 weeks to establish a multiple rectal exposure model of SIVmac 239 virus infection.Then, plasma viral load, CD4+ T cell count, T cell subsets and IFN-γsecretion of the experiment monkeys were determined . Results Low-dose SIVmac239 virus induced some changes in the immune system through the rectal mucosa , but didn’t induce typical infection.Repeated rectal mucosal low-dose virus exposure can activate the cellular immune system . Conclusions This study defines the effect of repeated low -dose simian immunodeficiency virus exposure on the systemic cellular immunity, and provided basic information for HIV-1 vaccine research.%目的：研究猴免疫缺陷病毒（SIVmac239）多次直肠粘膜暴露对机体细胞免疫的影响。方法建立小剂量多次直肠粘膜暴露模型，测定实验猴的血浆病毒载量了解病毒复制水平，测定外周 CD4＋ T 细胞绝对数了解疾病进展情况，测定 T 细胞亚群和外周单个核细胞 IFN-γ分泌情况了解机体细胞免疫状况。结果小剂量SIVmac239病毒多次暴露能导致病毒通过直肠粘膜进入动物体内，诱导机体免疫系统出现变化，但未见 SIVmac239典型感染。病毒多次直肠暴露诱导出特异性细胞免疫，但与普通病毒感染相比，水平较低。结论本研究明确了猴免疫缺陷病毒小剂量暴露对机体细胞免疫的作用，为 HIV 疫苗研究提供了基础信息。