DMBA／巴豆油二阶法联合 NB-UVB照射构建小鼠皮肤鳞癌模型

摘要

Objective To establish a mouse model of cutaneous squamous cell carcinoma induced by 7,12-dimethylbenz(a)anthracene (7,12-DMBA)/croton oil and narrow-band ultraviolet B (NB-UVB) irradiation.Methods A total of fifty 6-8-week old BALB/c mice (male:female 1:1) were randomly divided into three experimental groups.The group A was treated with chemical carcinogens alone, group B was treated with NB-UVB alone, and group C was treated with chemical carcinogens plus NB-UVB.The general status and skin appearance of mice were observed during the experiment.The survival rate and tumor formation rate of each group was calculated at weeks 5, 10, 15, and 20. Pathological examination was carried out to observe the histological changes of skin lesions.Results Papules measuring≥l mm in diameter began to develop in some mice of the group C at 5 weeks after the first treatment with chemical carcinogens.The tumor formation rates at 20 weeks after treatment were 86.67%, 7.14%, 94.12%in the groups A, B, C, respectively.Pathological examination revealed characteristic changes of squamous cell carcinoma in 13.34%, 0%, 70.59%of the mice in the group A, B, C, respectively.Conclusions Establishment of a mouse model of cutaneous squamous cell carcinoma induced by 7,12-DMBA/croton oil and NB-UVB is a better method than treated with chemical carcinogens alone or NB-UVB alone.This method can increase the tumor formation rate and incidence rate of SCC, and within a shorter period.%目的：用二甲基苯并蒽（ DMBA）／巴豆油二阶法联合NB-UVB 照射，构建皮肤鳞状细胞癌小鼠模型。方法将50只BALB／c小鼠，随机分为3组：A组外涂DMBA／巴豆油；B组照射NB-UVB；C组外涂DMBA／巴豆油，联合NB-UVB照射。实验过程中定期观察小鼠的一般情况和实验部位皮肤的变化，于第5、10、15、20周统计各组小鼠的存活率及成瘤率。第20周时取小鼠皮损组织进行病理检查。结果第5周末，部分C组小鼠出现直径≥1 mm的丘疹，第20周后，三组成瘤率分别为86.67％，7.14％，94.12％；SCC发生率分别为：13.34％，0％，70.59％。结论 DMBA／巴豆油二阶法联合NB-UVB构建小鼠皮肤鳞癌模型较单独应用DMBA／巴豆油二阶法或NB-UVB照射成瘤时间短，成瘤率和SCC发生率高。