Objective To explore a method to prepare a model of renal ischemia-reperfusion injury, with mild injury to animals, simple and easy to operate and stable effectiveness. Methods Healthy SD male rats were randomly divided into normal group (group C), sham-operated group (group S) and experimental group (IR group). Rats in the experimental group received a median incision in the middle of the back skin. Through the muscle fascia of each side on the back into the bilateral retroperitoneal space, the bilateral renal pedicles were separated, and closed for 50 min with a mini artery clamp without injury, and then the blood perfusion of the bilateral renal pedicles was resumed by releasing the mini artery clamp. Except that the rats of sham operation group were not blocked the renal pedicle, the rest of the operating steps were consistent with the experimental group. The normal group rats were not treated except for anesthesia. To observe the condition of the rats, serum creatinine (SCr), blood urea nitrogen (BUN) and pathologyical changes in the kidney tissues at 24 h after operating. Results Skin incision was 2. 24 ± 0. 27 cm in the group IR, the right incision of lumbar dorsal fascia and muscle was 1. 36 ± 0. 21 cm, and the left incision was 1. 36 ± 0. 24 cm. The operation time was 3. 30 ± 0. 37 min from incising skin to clamping the bilateral kidney pedicles. The successful rate of model preparation reached 95% in the IR group. Compared with the groups C and S, the levels of SCr and BUN were remarkably increased in the IR group (P ＜0. 01), and renal tubular necrosis scores were also significantly increased (P＜ 0. 05). Conclusions The rat model of renal ischemia-reperfusion injury is established by clamping bilateral renal pedicles via dorsal retroperitoneal incision. This surgical procedure is stable and simple, with a high success rate and small incision, less bleeding, and minimal animal injury.%目的 探索一种损伤小、操作简便且效果稳定的大鼠肾缺血再灌注损伤模型制作方法.方法 SD雄性大鼠随机分为正常组(C组)、假手术组(S组)、实验组(IR组).实验组大鼠经背部正中皮肤切口,通过两侧背部肌肉筋膜进入双侧腹膜后间隙,分离双侧肾蒂,用无损伤微型动脉夹夹闭双侧肾蒂50 min后再松开,恢复灌流.假手术组大鼠不夹闭肾蒂,其余步骤与实验组一致;正常组大鼠仅做麻醉.观察术后24 h大鼠生存状态、血肌酐、尿素氮和肾组织结构变化.结果 IR组皮肤切口(2. 24 ± 0. 27) cm,右腰背部筋膜与肌肉切口(1. 36 ± 0. 21) cm,左侧腰背部筋膜与肌肉切口(1. 36 ±0. 24)cm.从切开皮肤至夹闭双肾蒂用时(3. 30 ±0. 37)min. IR组造模成功率95% .与正常组、假手术组比较,IR组血肌酐、血尿素氮水平显著性增高(P＜ 0. 01),肾小管损伤评分明显升高(P＜ 0. 05).结论 采用背入式腹膜后双肾蒂夹闭法建立肾缺血再灌注损伤模型,效果稳定,成功率高,切口小,出血少,对动物刺激轻,易操作.
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