复方苦参注射液诱导胃癌细胞株MGC803增殖抑制和细胞凋亡的实验研究

摘要

Objective: To investigate the inhibitory effects and possible mechanisms of matrine injections on the proliferation and apoptosis of human gastric cancer cell line MGC803.Methods: After matrine injections, cell proliferation was determined by the MTT assay.Cell morphology and apoptosis quantity were examined by the terminal deoxynucleotidyl transferase dUTP nick end labeling ( TUNEL ) assay.The expression levels of Bcl-2, Bax, and Caspase-3 proteins were observed by Western blot analysis.Results: Matrine injections significantly inhibited the proliferation of MGC803 cells in concentration- and time-dependent manners ( P ＜ 0.001).The number of apoptotic cells in the matrine-treated group was greater than that of the control group ( P ＜ 0.05 ).The expression level of Bcl-2 was down-regulated by matrine.With increased matrine concentration, the ratio of Bcl-2 to Bax decreased, consequently accelerating procaspase-3 activation ( P ＜ 0.05 ).Conclusion: Matrine injections inhibited the growth of MGC803 cells in concentration- and time-dependent manners by inducing apoptosis.This action may be mediated by the down-regulation of Bcl-2/Bax, resulting in Caspase-3 activation.%目的:观察复方苦参注射液在体外抑制人胃癌细胞株MGC803增殖和诱导其凋亡的生物学效应,并对其分子机制作初步探讨.方法:分别采用不同浓度梯度的复方苦参注射液干预胃癌细胞MGC803,通过MTT比色法检测复方苦参注射液对该细胞系生长增殖的影响.TUNEL法分析经1、2、4 mg/mL复方苦参注射液作用24 h后的MGC803细胞凋亡率.Western blot法检测凋亡相关基因Bcl-2、Bax、Caspase-3蛋白表达情况.结果:复方苦参注射液在体外能明显抑制人胃癌细胞株MGC803的增殖,且呈浓度、时间依赖性(P<0.001).TUNEL法检测实验组细胞凋亡率均明显高于未加复方苦参注射液的空白对照组(P<0.05);West?ern blot分析表明复方苦参注射液能下调Bcl-2蛋白表达,降低蛋白Bcl-2/Bax比值,并且可以促使Caspase-3原蛋白发热活化(P<0.05).结论:复方苦参注射液具有体外抑制胃癌细胞增殖的作用,并能诱导其凋亡,其机制可能与Bcl-2/Bax比值降低导致Cas?pase-3活化相关.