锌指蛋白185对人胶质瘤细胞迁移的影响

摘要

目的：探讨锌指蛋白185（zinc finger protein 185，ZNF185）在人胶质瘤细胞迁移中的作用。方法：Western blot检测ZNF185在神经胶质瘤及瘤旁正常组织的蛋白表达，克隆ZNF185基因转染人胶质瘤细胞，免疫荧光染色检测ZNF185的细胞定位，细胞划线和Transwell小室法检测ZNF185蛋白对细胞迁移的影响。结果：与瘤旁正常脑组织相比，ZNF185在人胶质瘤组织和细胞系中的表达显著降低；ZNF185蛋白主要分布于人胶质瘤细胞的细胞质、细胞膜，并与肌动蛋白纤维共定位；过表达ZNF185显著抑制人胶质瘤细胞的迁移。结论：ZNF185通过结合肌动蛋白纤维发挥抑制人胶质瘤细胞迁移的作用。%Objective:To explore the effect of Zinc finger protein 185 (ZNF185) on the migration of human gliocytoma cells. Methods: ZNF185 protein expression in human gliocytoma and its adjacent normal tissues was detected through Western blot. The ZNF185 gene was cloned and transfected into the human gliocytoma cell line SF767. ZNF185 cells were located through immunofluo-rescence staining, and cell migration was analyzed using the cell scoring method. Results:CZNF185 protein expression was lower in the human gliocytoma cell line than in normal para-neoplastic tissues. ZNF185 protein was mainly expressed in the cytoplasm and cell membrane of the human gliocytoma cells and was co-located with F-actin. ZNF185 overexpression repressed the migration of human gliocytoma cells. Conclusion:ZNF185 represses the migration of human gliocytoma cells by binding to F-actin.