IL-6-174G＞C基因多态性与南京地区汉族人群冠心病的相关性

摘要

目的 探讨IL-6基因启动子区域-174G＞C基因多态性与南京汉族人群冠心病(CHD)的关系.方法 聚合酶链-限制性片段多态性(PCR-RFLP)检测235例CHD患者和175例体检健康者,分析各组IL-6-174G＞C基因多态性,并进行Hardy-Wein-berg平衡检验,选取部分DNA样本进行基因测序,以验证其基因型.同时检测各组血脂、Glu、C反应蛋白、糖化血红蛋白的表达水平.结果 CHD组和健康人对照组GG、GC、CC基因型频率分别为98.7％、1.3％、0和97.7％、2.3％、0,G、C等位基因频率分别为99.4％、0.6％和98.9％、1.1％,基因型和等位基因频率在两组间的分布差异无统计学意义(P均＞0.05),与健康人对照组相比,CHD组吸烟、SBP、DBP、TG、CHOL、HDL-C、ApoA-I、ApoB、CRP、HbAlc差异均有统计学意义(P均＜0.05),而年龄、性别、Glu差异均无统计学意义(P均＞0.05).结论 IL-6-174G＞C基因多态性与南京地区汉族人群CHD没有明显的相关性.%Objective To investigate the relationship of-174G ＞ C polymorphism of interleukin 6 (IL-6) gene promoter region upstream-with coronary heart disease (CHD) of Chinese Han population in Nanjing area.Methods The polymorphism of IL-6-174G ＞C gene was confirmed in 235 patients with CHD and 175 healthy individuals by PCR-RFLP,and Hardy-Weinberg equilibrium was tested.DNA samples were selected for sequencing to verify their genotype.The concentration of blood sugar,lipid,C reaction protein (CRP) and glycosylated hemoglobin (HbAlc) in the samples were measured simultaneously.Results The genotype distributions of GG,GC and CC were 98.7％,1.3％ and 0 in CHD group and 97.7％,2.3％ and 0 in control group,respectively.The frequencies of G and C alleles were 99.4％,0.6％ and 98.9％,1.1％ in the two groups.There were no statistical significance for frequencies of genotype and alleles between the two groups (all P ＞ 0.05).Compared with the control group,the differences of smoking,systolic blood pressure (SBP),diastohc blood pressure (DBP),triglyceride (TG),cholesterol (CHOL),low-density lipoproteins-cholesterol (LDL-C),high-density lipoproteins-cholesterol (HDL-C),apolipoprotein A-I (ApoA-I),ApoB,CRP and HbA1 c were statistically significant (all P ＜ 0.05),while age,sex and blood glucose were not statistically significant (all P ＞ 0.05).Conclusion IL-6-174 G ＞ C gene polymorphism should not remarkably correlated with CHD in Chinese Han population in Nanjing area.