阿司匹林对铜绿假单胞菌生物膜形成和分散的影响

摘要

目的 探讨阿司匹林对铜绿假单胞菌生物膜的形成和分散的作用.方法 微量肉汤稀释法检测铜绿假单胞菌浮游菌对阿司匹林的敏感性;通过微孔板构建生物膜,结合结晶紫染色探讨阿司匹林对铜绿假单胞菌生物膜的形成和分散的影响;通过微孔板结合稀释平板计数法检测阿司匹林对铜绿假单胞菌初始粘附能力的影响.结果 铜绿假单胞菌标准菌株PAO1和临床菌株PA18、PA53和PA67对阿司匹林MIC值分别为5 mg/mL、2.5 mg/mL、5 mg/mL和5 mg/mL.当阿司匹林浓度为2.5 mg/mL时可显著抑制PAO1和PA18生物膜的形成(t=5.65,P＜0.05;t =5.06,P＜0.05),当其浓度分别为1.25 mg/mL和0.313 mg/mL时能显著抑制PA67和PA53生物膜的形成(t=4.10,P＜0.05;t=5.12,P＜0.05).2.5 mg/mL阿司匹林可显著分散PAO1和PA18培养24h形成的生物膜(t=6.45,P＜0.05;t =6.26,P＜0.05),1.25 mg/mL和0.313 mg/mL时可显著分散PA67和PA53形成的生物膜(t=7.82,P＜0.05;=9.18,P＜0.05).2.5 mg/mL阿司匹林能显著抑制铜绿假单胞菌的初始粘附(P＜0.05).结论 阿司匹林能有效抑制铜绿假单胞菌的初始粘附能力和生物膜的形成,并对已形成生物膜具有分散能力.%Objective To explore the effects of aspirin on formation and dispersion of Pseudomonas aeruginosa (P.aeruginosa) biofilm.Methods The broth microdilution method was used to detect the minimal inhibitory concentration(MIC) of aspirin for P.aeruginosa.The anti-biofilm effects of aspirin on P.aeruginosa were determined on the microtiter plates combined with crystal violet staining.The serial dilution method for counting colony number on microtiter plate was used to explore the effects of aspirin on initial adherence of P.aeruginosa.Results The MIC values of aspirin against PAO1,PA18,PA53 and PA67 strains of Pseudomonas aeruginosa were 5,2.5,5 and 5 mg/mL respectively.Aspirin significantly inhibited the formation and dispersion of the biofilm of PAO1 and PA18 strains (t =5.65,P ＜ 0.05 and t =5.06,P ＜ 0.05 for inhibition;t =6.45,P ＜ 0.05 and t =6.26,P ＜ 0.05 for dispersion) at the concentration of 2.5 mg/mL.Similar effects were also found in the determination for PA67 and PA53 strains(t =6.45,P ＜0.05 and t =6.26,P ＜ 0.05 for inhibition;t =7.82,P ＜ 0.05;t =9.18,P ＜ 0.05 for dispersion) at aspirin concentration of 1.25 and 0.313 mg/mL respectively.Aspirin inhibited the initial adherence of P.aeruginosa at the concentration of 2.5 mg/mL(P ＜0.05).Conclusion Aspirin could significantly inhibit the initial adherence and biofilm formation of P.aeruginosa and disperse the 24 hour-formed mature bioiflm.