罕见B(A)血型的鉴定

摘要

Objective To investigate the serological and molecular identification of 2 rare B( A) blood groups. Methods The ABO blood groups of 2 samples from blood donors were detected by routine serological method. The genotype features was identified by PCR-sequence specific primer (PCR-SSP) and direct sequence analysis. Results The serological results for the 2 blood donors showed the characteristics of B(A) phenotype. The sample 1 was genotyped as BO2 subtype by PCR-SSP and direct sequencing showed B alleles in exon 7, presented nt640 A＞G mutation which was confirmed to be B(A)04/O02 genotype.The sample 2 was genotyped as BO1 sub-type by PCR-SSP and direct sequencing showed B alleles presented nt700 C＞G mutation in exon 7 which was confirmed to be B(A)02/O01 genotype. Conclusion The phenotype of the two samples should be B ( A ) and the genotypes should be rare B(A)04/O02 and B(A)02/O01.%目的 观察2例罕见B(A)血型的血清学特征并研究其分子机制.方法 用血型血清学鉴定2例献血者标本ABO血型,用序列特异性引物聚合酶链反应(PCR-SSP)基因分型和直接测序确定其基因型.结果 2例献血者血型血清学检测结果均表现为B(A)亚型的特点.标本1基因分型为BO2,测序结果为第7外显子B基因发生640A＞G突变,符合B(A)04/O02的基因型特点;标本2基因分型为BO1,测序结果为第7外显子B基因发生700C＞G突变,符合B(A)02/O01的基因型特点.结论 2例标本均为B(A)表现型,基因型分别为B(A)04/O02和B(A)02/O01.