卵巢浆液性腺癌组织中TK1和Ki-67表达的意义

摘要

Purpose To study the significance of the expression of TK1 and Ki-67 in ovarian serous adenocarcinoma. Methods The clinicopathological characteristics were retrospectively reviewed in 55 cases of ovarian serous adenocarcinoma. All the cases were chosen for immunohistochemical staining with antibodies against TK1 and Ki-67 proteins, and the clinical significance of these immunohis-tochemical markers analyzed. Results The positive rates of TK1 and Ki-67 in ovarian serous adenocarcinoma were 72. 7% and 80.0% , respectively. The positive expression of TK1 localized in the cytoplasm, and Ki-67 localized in the nucleus. TK1 expression was correlated with the maximum tumor diameter, pTNM, recurrence and pathological grade ( P < 0. 05 ). There was statistically correlation between the expression of Ki-67 with pTNM stage, recurrence and pathological grade ( P <0. 05 ). Furthermore, kappa consistency test showed the expression of TK1 had good consistency with recurrence ( P < 0. 05 ), and owed more superior value for determining the recurrence than Ki-67. By the followed-up data of 55 cases, Kaplan-Meier analysis showed that pTNM stage, recurrence, pathological grade and the expression of TK1 and Ki-67 were all related to the prognosis ( P < 0. 05 ). Multivariate analysis displayed only the recurrence retained significance in prospecting prognosis. Conclusion The prognosis of ovarian serous adenocarcinoma is most closely related with tumor recurrence, and TK1 has the reference value for judging the tumor recurrence. High expression of TK1 indicates that there is high likelihood of recurrence, suggesting that intensive therapy and frequent follow-up should be performed.%目的 探讨细胞质胸苷激酶1(cytoplasmic thymidinekinas-1,TK1)、Ki-67在卵巢浆液性腺癌中表达的意义.方法 回顾性研究55例经手术治疗的卵巢浆液性腺癌患者的临床病理资料,并运用免疫组化技术观察TK1、Ki-67在卵巢浆液性腺癌中的表达情况及与临床病理参数之间的意义.结果 TK1阳性表达定位于细胞质,阳性率为72.7%.TK1的表达与肿瘤的最大径、复发、pTNM分期、病理分级密切相关(P＜0.05).Ki-67阳性表达定位于细胞核,阳性率为80.0%,Ki-67的表达与肿瘤的复发、pTNM分期、病理分级有关(P＜0.05).Kappa检验显示TK1的表达与卵巢浆液性腺癌复发较一致(k=0.559,P=0.000),且判断复发比Ki-67更为优越.Kaplan-Meier检验显示pTNM分期、肿瘤复发、MDACC分级、Ki-67、TK1表达分别与预后有关(P＜0.05).COX回归多因素分析显示:肿瘤复发是影响卵巢浆液性腺癌患者的独立性预后因素.结论 卵巢浆液性腺癌的复发影响患者的预后,TK1对判断卵巢浆液性腺癌是否有复发倾向具有参考价值,且优于Ki-67,初次手术后肿瘤组织免疫组化TK1高表达预示患者存在高复发的风险,提示临床要加强术后的治疗方案,并提高随访频数.