甲状腺乳头状癌中MCM7、CDK2、p27蛋白的表达及意义

摘要

目的 探讨MCM7、CDK2及p27蛋白与人甲状腺乳头状癌(papillary thyroid carcinoma,PTC)发生、发展的关系.方法 采用免疫组织化学SP法检测40例PTC、30例甲状腺腺瘤、30例结节性甲状腺肿及20例正常甲状腺组织中MCM7、CDK2、p27蛋白的表达.结果 PTC中MCM7、CDK2蛋白的阳性表达率分别为100.00%(40/40)、80.00%(32/40),两者均明显高于甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织(P<0.01,P<0.01).PTC中p27蛋白的阳性表达率为22.50%(9/40),明显低于甲状腺腺瘤、结节性甲状腺肿及正常甲状腺组织(P<0.01).PTC中MCM7与CDK2蛋白的表达呈正相关(r=0.550,P<0.01),MCM7、CDK2及p27蛋白的表达呈负相关(r=-0.334,P<0.05;r=-0.413,P<0.01).结论 MCM7、CDK2蛋白的高表达及p27蛋白的低表达变化与PTC可能存在关联,三者联合检测或许可以作为临床早期诊断和评价甲状腺肿瘤细胞增殖活性的潜在的参考指标.%Purpose To explore the expression of MCM7 , CDK2 and p27 in papillary thyroid carcinoma and the mechanisms involved in the carcinogenesis and development of papillary thyroid carcinoma.Methods Immunohistochemical SP method was used to detect the expression of MCM7 , CDK2 and p27 protein in 40 cases of papillary thyroid carcinoma, 30 cases of thyroid adenoma.30 cases of nodular goitre and 20 cases of normal thyroid gland tissue.Results The positive rates of MCM7 and CDK2 expressions in papillary thyroid carcinoma were 100.00％( 40/40 ) and 80.00％ (32/40 ).The expression of MCM7 and CDK2 protein in the papillary thyroid carcinoma group was significantly higher than that in the thyroid adenoma.nodular goitre and normal thyroid tissue groups ( P ＜ 0.01 ,P ＜ 0.01 ).The positive rates of p27 expressions in papillary thyroid carcinoma were 22.50％( 9/40 ).The expression of p27 protein in the papillary thyroid carcinoma group was significantly lower than that in the thyroid adenoma.nodular goitre and normal thyroid tissue groups ( P ＜ 0.01 ).A negative correlation was ohserved between the expressions of MCM7 and p27 protein in papillary thyroid carcinoma tissue ( r = - 0.334, P ＜ 0.05 ).A negative correlation was ohserved between the expressions of CDK2 and p27 protein in papillary thyroid carcinoma tissue ( r = - 0.413 , P ＜ 0.01 ).A positive correlation was observed between the expressions of MCM7 and CDK2 protein in papillary thyroid carcinoma tissue ( r = 0.550, P ＜ 0.01 ).Conclusions The high expression of MCM7 and CDK2 protein and low expression of p27 protein may be involved in and the carcinogenesis of papillary thyroid carcinoma.Combined detecting of MCM7 , CDK2 and p27 protein may he used as a reference index in the early diaynoses and determine the hiological indicators of cell proliferation of papillary thyroid carcinoma.