胃腺癌中p57kip2和LIMK-1的表达及意义

摘要

Purpose To explore the expression of p57kip2, LIMK-1 in gastric adenocarcinoma tissues, and to analyze their correlation between the expression of p57kip2, LIMK-1 and tumor differentiation and biological behavior. Methods The expressions of p57kip2 and LIMK-1 protein and their mRNA in 40 cases of gastric adenocarcinoma were detected by EnVision immunohistochemistry and digoxigenin labeled oligonucleotide probe in situ hybridization, respectively. Results ( 1 ) Expression of p57kip2 in normal gastric mucosa was higher than that in the cancerous tissues, the difference was statistically significant ( P <0. 05 ). ( 2 ) The relationship between the expression of p57kip2 and histological grade were negatively correlated in gastric cancer ( P <0. 05 ). ( 3 ) Expression of p57kip2, LIMK-1 was positively correlated with LVD and MVD in gastric adenocarcinoma ( P <0. 05 ). Conclusion The low expression of P57kip2and the high expression of LIMK-1 may be important biological markers of malignant transformation of gastric mucosa and the lymph node metastasis of gastric adenocarcinoma, and it is important in predicting prognosis, invasion and metastasis of the gastric adenocarcinoma.%目的 探讨p57kip2mRNA、LIMK-1mRNA及其蛋白在胃腺癌组织中的表达,了解其与肿瘤组织分化程度及临床病理特征的关系.方法 采用显色原位杂交(chromogenic in situ hybridizations,CISH)技术和免疫组化EnVision法检测40例胃腺癌及癌旁正常胃黏膜组织中p57kip2mRNA、LIMK-1mRNA及其蛋白的表达.结果 (1)正常胃黏膜组织中p57kip2高表达,在胃腺癌组织中低表达,差异具有统计学意义(P<0.05);(2)p57kip2的表达与胃腺癌组织分化程度呈负相关(P<0.05);(3)p57kip2、LIMK-1蛋白表达与胃腺癌组织中微脉管计数微淋巴管密度(lymphaticmicrovessel density,LVD)、微血管密度(microvesseldensity,MVD)呈正相关(P<0.05).结论胃腺癌存在p57kip2基因组印记缺失,致p57kip2mRNA及其蛋白表达减少或缺失,并与胃腺癌组织分化程度有关,LIMK-1蛋白高表达与肿瘤的浸润转移及微脉管的生成有关,检测胃癌活检标本中LIMK-1蛋白的表达,对预测胃腺癌预后及浸润转移有辅助诊断意义.