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首页> 外文期刊>中国化学工程学报（英文版） >酵母细胞FD11b生物不对称公式(R)-(-)-扁桃酸的动力学研究
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酵母细胞FD11b生物不对称公式(R)-(-)-扁桃酸的动力学研究

机译：酵母细胞FD11b生物不对称公式(R)-(-)-扁桃酸的动力学研究

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机译：The kinetics of asymmetric production of R-(-)-mandelic acid (R-MA) from phenylglyoxylic acid (PGA)catalyzed by Saccharomyces cerevisiae sp. strain FD11b was studied by fed-batch cultures. The concentrations of glucose and PGA were controlled respectively with a dual feeding system. When the electron donor glucose was supplied at the rate of 0.0833mmol·gdw-1·h-1, the specific production rate (qp) and the enantiomeric excess of R-MAreached the maximum 0.353mmol·gdw-1·h-1 and 97.1％, respectively. The apparent reduction activity of yeast FD11b was obviously affected by both substrate PGA and product MA. The qp value reached the maximum 0.36-0.38mmol·gdw-1·h-1 when the PGA concentration was controlled between 25 and 35mmol·L-1. The obvious substrate inhibition of bioconversion was observed at the PGA concentrations higher than 40mmol· L-1. The accumulation of product MA also caused a severe feed-back inhibition for its production when the product concentration was above 60mmol· L-1. The kinetic model with the inhibition effect of both substrate and product was simulated by a computer-based least-square arithmatic. The established kinetic model was in good agreement with the experimental data.

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