快速起搏大鼠原代培养心房肌细胞内皮素-1表达的变化

摘要

目的:利用原代培养的心房肌细胞建立快速起搏模型,研究在快速起搏早期内皮素-1(ET-1)表达的变化.方法:以原代培养大鼠心房肌细胞,建立快速起搏细胞模型,利用酶联免疫吸附(ELISA)法和逆转录聚合酶链式反应(RT-PCR)方法检测在快速起搏3、6、12、24h后内皮素-1的mRNA表达和分泌量的变化.结果:与起搏前比较,快速起搏6h后ET-1的mRNA表达[(0.31±0.02)比(0.52±0.07)、(0.62±0.09)、(0.75±0.05)]和分泌量[(3.4±0.8)ng/L比(6.0±1.4)ng/L、(8.3±1.5)ng/L、(11.2±2.1)ng/L]持续增加(P均＜0.01).结论:快速起搏早期,原代培养心房肌细胞内皮素-1的mRNA表达和分泌量随起搏时间增加,提示内皮素-1参与了房颤时心房病理重构.%Objective:To study the expressions of endothelin-1 in primary cultured atrial myocyte model at early stages of atrial fibrillation by rapid pacing.Methods:The primary rat atrial myocytes were cultured,in which a rapid paced cell model was established.The polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were applied to detect the messenger ribonucleic acid (mRNA) expression and secretion of endothelin1 on 3h,6h,12h and 24h after rapid pacing.Results:Compared with before pacing,there were continuous and significant increase in expression of ET-1mRNA [ (0.31±0.02) vs.(0.52±0.07),(0.62±0.09),(0.75±0.05)] and secretion of endothelin-1 [ (3.4±0.8) ng/L vs.(6.0±1.4) ng/L,(8.3±1.5) ng/L,(11.2±2.1) ng/L] on 6h,12h and 24h after rapid pacing,P＜0.01 all.Conclusion:Expressions of ET-1mRNA and secretion of endothelin-1significantly increase depend on time in early rapid pacing atrial myocytes,indicating that endothelin-1 participates in atrial pathological remodeling during atrial fibrillation.