Analysis of contaminants biotoxin mussels, clams and oysters grown, using primary cultures of rat cerebellar neurons.

摘要

ANALYSIS OF CONTAMINANTS BIOTOXINS mussels, clams and OYSTERS GROWN USING PRIMARY CULTURES OF NEURONS rat cerebellar, DEALING WITH THESE DUPLICATE SAMPLES EXTRACT mollusk. The toxicity of each extract was evaluated from neurotoxic effects caused by FOUR dilutions thereof: 0, 10, 50 and 100, FOR A MERGER OF biotoxins, okadaic acid and domoic acid, superiors, peers and below its limit LEGAL IN TISSUE mollusk. The analysis is performed in presence of 2X10BS-6 SC M MK801 SEPARATELY FOR VIEWING okadaic acid neurotoxicity AND domoic. TOXICITY OF EACH EXTRACT IS EVALUATED CROPS TO OBSERVING phase contrast microscopy after 30 '' TREATMENT FOR domoic acid and okadaic 24H for acid. TOXICITY can be checked by staining COMBINATION OF VITAL DYE neurons with a fluorescent ethidium bromide.