灌流速度与新西兰兔心肌细胞急性分离的关系

摘要

Objective:To establish a simple,stable and high success rate separation method of myocardial cells in New Zealand rabbits.Methods:Compared influence between perfusion speed of 50 r/min (8 ml/min) and 65 r/min (11ml/min) of Langendorff perfusion device on cell separation during separation course of myocardial cells.Results:Compared with perfusion speed of 50 r/min,there was significant decrease in time to obtain cells [(33±9.10) min vs.(19.29±2.73) min],and significant increase in success rate (50％ vs.91％) and survival rate [(38.26±2.63)％ vs.(85.71±2.21)％] by perfusion speed of 65 r/min,P＜0.01 all.Conclusion:Perfusion speed is an important factor that affects acute separation of myocardial cells in New Zealand rabbits,and 65r/min perfusion speed may be more stable and successful to obtain single myocardial cell.%目的:建立一种简单,稳定,成功率高的新西兰兔心肌细胞分离方法.方法:比较分离细胞过程中Langendorff灌流装置的灌流速度[50r/min (8ml/min)和65r/min(11ml/min)]对细胞分离的影响.结果:与灌流速度为50r/min时比较,灌流速度为65r/min获得细胞所需时间[(33±9.10) min比(19.29±2.73) min]明显缩短,成功率(50％比91％)、存活率[(38.26±2.63)％比(85.71±2.21)％]明显提高(P均＜0.01).结论:灌流速度是影响新西兰兔心肌细胞急性分离的重要因素,65r/min灌流速度能更稳定、成功获取单个心肌细胞.