妊娠高血压IL-2、IL-10分泌异常对Th1/Th2的影响

摘要

目的:探讨妊娠期高血压患者白细胞介素(IL)-2、IL-10水平与Th1/Th2型细胞含量比值及平均动脉压的相关性.方法:选择我科住院的正常孕妇(50例,正常对照组)和妊高症患者(47例,妊高症组)为研究对象.采用放射免疫分析法检测两组IL-2、IL-10水平,流式法检测Th1、Th2,计算Th1/Th2值,并进行比较分析.采用Spearman相关分析IL-2、IL-10水平与Th1、Th2、平均动脉压的相关性.结果:与正常对照组比较,妊高症组IL-2[(0.79 ± 0.14)pg/ml比(1.64 ± 0.32)pg/ml]、Th1[(8.95 ± 0.78)% 比(16.02 ± 1.43)%]、Th1/Th2值[(8.46 ± 0.49)比(17.95 ± 1.24)]及平均动脉压[(80.91 ± 10.23)mmHg比(134.7 ± 26.4)mmHg]明显升高, IL-10[(129.46 ± 28.61)pg/ml比(64.25 ± 9.86)pg/ml]、Th2[(1.09 ± 0.12)% 比(0.87 ± 0.05)%]水平明显降低(P均=0.001).IL-2水平与Th1、Th1/Th2、平均动脉压呈明显正相关(r=0.493~0.618,P<0.05或<0.01),与Th2呈明显负相关(r= -0.359,P=0.024);IL-10水平与Th1、Th1/Th2、平均动脉压呈明显负相关(r= -0.694~ -0.518,P<0.05或<0.01),与Th2呈明显正相关(r=0.458,P=0.014).结论:妊高症患者的免疫因子IL-2、IL-10分泌异常时能使Th1/Th2失衡,且与高血压有明确关系,影响高血压进程和转归.%Objective:To explore correlation among levels of interleukin(IL)-2,IL-10 and Th1/Th2 ratio,mean arterial pressure(MAP)in patients with gestational hypertension(GH).Methods:A total of 50 normal pregnant women(normal control group)and 47 GH patients(GH group)hospitalized in our hospital were selected.Levels of IL-2 and IL-10 were measured by radioimmunoassay,Th1 and Th2 were measured by flow cytometer,then Th1/Th2 was calculated,and above indexes were compared and analyzed between two groups.Spearman correlation analysis was used to analyze the correlation among IL-2,IL-10 levels and Th1/Th2,MAP.Results:Compared with normal control group,there were significant rise in IL-2 level[(0.79 ± 0.14)pg/ml vs.(1.64 ± 0.32)pg/ml],Th1[(8.95 ± 0.78)% vs.(16.02 ± 1.43)%],Th1/Th2 ratio[(8.46 ± 0.49)vs.(17.95 ± 1.24)]and MAP[(80.91 ± 10.23)mmHg vs.(134.7 ± 26.4)mmHg],and significant reductions in levels of IL-10[(129.46 ± 28.61)pg/ml vs.(64.25 ± 9.86)pg/ml]and Th2[(1.09 ± 0.12)% vs.(0.87 ± 0.05)%]in GH group,P=0.001 all.IL-2 level was significant positively correlated with Th 1,Th1/Th2 and MAP(r=0.493~0.618,P<0.05 or <0.01),and significant inversely correlated with Th 2(r= -0.359,P=0.024);IL-10 level was significant inversely correlated with Th 1,Th1/Th2 and MAP(r= -0.694~ -0.518,P<0.05 or <0.01),and significant positively correlated with Th 2(r=0.458,P=0.014).Conclusion:Immune factors IL-2 and IL-10 secretion ab-normalities in GH patients can lead to Th1/Th2 imbalance,and they are closely correlated to hypertension,which affect progress and outcome of hypertension.