螺旋纤维床固定化生物反应器同时产酶降解壳聚糖的研究

摘要

The cells of Trichoderma reesei were immobilizxed on a roll of porous polyurethane foam sheet and packed in a bubble column bioreactor for simultaneous production of chitosanase and degradation of chitosan in situ. The average degree of polymerization could be regulated by reaction time. Under the repeated-batch process with 2 % soluble chitosan at pH4.8,28℃ , the activity of chitosanase for each batch was above 0.15 IU/mL, the average yield of reducing sugar as D-glucosamine reached 73%. The novel immobilized bioreactor system run stably and effectively in the successive 10 batches lasting 30 days without notable change in the activity and productivity.%采用多孔聚酯泡沫固定里氏术霉，在鼓泡柱固定化反应器中同时产酶降解壳聚糖。结果表明通过控制降解时间可以得到不同平均聚合度的降解产物。在28℃，pH4.8，通气量3vvm条件下，利用固定化反应器，在30d内连续进行10批同时产酶降解试验，结果发现壳聚糖酶活力和壳聚糖降解率能保持稳定。每批产生的壳聚糖酶活力平均达到0.15u／mL以上，壳聚糖平均降解率为73％。