中高温油藏内源微生物好氧激活技术研究

摘要

In order to study the endogenous microbial aerobic activation characteristics in middle and high temperature reservoir,12 typical blocks in high temperature reservoirs in Shengli Oilfield were selected to carry out the aerobic activation evaluation.Three reservoir temperature range were selected,namely from 55 to 65 ℃,65 to 79 ℃ and 79 to 95 ℃.6 different carbon sources were selected as the main activator:different activator formulation was prepared.Under the simulated reservoir conditions of aerobic activation evaluation,we found that the additional nutrient agent of corn syrup, molasses and yeast powder could effectively activate the functional strain below 79 ℃ oil reservoir blocks,the cell density could reach 3× 109 cell/mL.The emulsifying and dispersing effect of crude oil was obvious. In oil reservoir blocks above 79 ℃,the activation effect and emulsifying effect of crude oil were generally worse than that in 79 ℃ oil reservoir blocks.The cell density and emulsifying effect after activation show a positive correlation in each block center,whereas the surface tension and emulsifying effect had no obvious correlation. The surface tension of the solution after aerobic activation was reduced from 65 mN/m to 47 mN/m. The dominant bacteria after aerobic activation were mainly Geobacillus and Bacillus thermoamylovorans.%为了掌握中高温油藏内源微生物好氧激活特征，在胜利油田选取12个中高温油藏典型区块开展了好氧激活评价：在所选油藏温度范围分别为55～65℃、65～79℃、79～95℃，通过模拟油藏条件下好氧激活评价发现：在小于79℃油藏区块中，以玉米浆干粉、糖蜜、酵母粉等作为外加营养剂能够有效激活水样中的功能菌株，激活后菌密度最高能够达到3×109个／mL，原油乳化分散作用明显；在大于79℃的油藏区块中，激活效果和原油乳化效果普遍比小于79℃的油藏区块差。各区块中心井激活后的菌密度与乳化效果呈现出一定的正相关性，而表面张力与乳化效果无明显对应关系，好氧激活后的溶液表面张力由65 mN／m降至47 mN／m。好氧激活后形成的优势菌主要以地衣芽胞杆菌（ Geobacillus）和热嗜淀粉芽胞杆菌属（ Bacillus thermoamylovorans）为主。