枯草芽胞杆菌M15101强化内源微生物驱油模拟实验

摘要

以分离自油藏环境的产脂肽枯草芽胞杆菌M15101为研究对象，探究了外源表面活性剂产生菌强化油藏内源微生物驱油过程中的原油乳化分散效果和菌群间的相互作用。配伍性实验表明，该菌株与油藏常见采油功能菌———迪茨氏菌、红球菌和肠杆菌具有良好的配伍性。荧光定量PCR和高通量测序分析结果表明，枯草芽胞杆菌M15101对内源菌群结构无不利影响。摇瓶激活实验和气象色谱分析结果显示，枯草芽胞杆菌M15101能够显著改善内源菌群激活初期油相乳化分散效果，促进原油降解，为后续广泛应用于现场试验提供基础。%We used Bacillus subtilis M15⁃10⁃1 as the research object. Bacillus subtilis M15⁃10⁃1 is separated from the reservoir environment and can produce lipopeptide. We explored the exogenous surfactant producing bacteria to strengthen endogenous microbial flooding reservoirs in the process of crude oil emulsion dispersion effect and the interaction among bacterial flora. Compatibility experiments showed that Bacillus subtilis M15⁃10⁃1 and the oil reservoir functional bacteria, Dietzia sp. ZQ⁃4, Rhodococcus sp. M, and Enterobacter cloacae T⁃1 has good compatibility. Fluorescence quantitative PCR and high⁃throughput sequencing analysis showed that Bacillus subtilis M15⁃10⁃1 was the structure of reservoir indigenous microorganism without adverse impact. Activation experiment in laboratory and meteorological chromatography analysis showed that Bacillus subtilis M15⁃10⁃1 could significantly improve the oil water activation in early oil phase emulsifying dispersion effect and promote the petroleum hydrocarbon degradation.This method provides the foundation for subsequent field test.