The aim of this work is to explain the mechanisms of electrophysiological changes in rat blood cell by using RC equivalent circuit model under simulated weightlessness. Simulating weightlessness model was established by rat tail suspension. Electrical impedance spectroscopy of rat blood on the control and tail suspension group was performed using Aligent 4294A impedance analyzer. Electrical parameters ( resistivity and capacitance) of blood were compared between the control and tail suspension through numerical calculation of equivalent circuit model and curve fitting analysis. After 60 days post simulating weightlessness, decrease in the hematocrit ( Hct) and resistivity of the erythrocyte membrane were compared with that of normal group. Experimental reuslts showed that the decrease in extracellular and intracellular resistivity was 16. 44% and 1.54% respectively, the cell membrane and intracellular capacitance decreased by 4.66% and 0.83% respectively, but extracellular capacitance remained unchanged. The rat blood hematocrit ( Hct ) and erythrocyte membrane resistivity were significantly reduced in the simulating weightlessness model, which led to reduction in electrical impedance of the extracellular fluid and whole blood, thus gave rise to electrical conductivity. The electrical impedance spectroscopy moved to low resistance direction.%利用RC等效电路模型解析模拟失重引起大鼠血液变化的电生理机制.采用大鼠尾部悬吊方法建立模拟失重动物模型,使用Aligent 4294A阻抗分析仪测量对照组与模拟失重大鼠血液电阻抗谱,通过血液等效电路模型的数值计算和曲线拟合,比较、分析对照组与模拟失重组血液电学参数(电阻和电容).结果表明,模拟失重60d后,与对照组比较,失重大鼠血液的红细胞比容(Hct)降低,细胞膜电阻率减少,细胞外和细胞内电阻率分别减少16.44％和1.54％,细胞膜和细胞内电容分别减少4.66％和0.83％,细胞外电容不变.模拟失重使大鼠血液的红细胞比容(Hct)明显降低,红细胞膜电阻率显著降低,导致细胞外液和全血电阻抗降低,导电性增加,电阻抗谱向低阻方向移动.
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