转基因大豆的环境安全性评价对我国转基因大豆产业发展具有重要的理论和实践生产意义.本研究以转入G2-EPSPS和GAT双价基因抗草甘膦大豆ZH10-6及其非转基因大豆中黄10为研究试材,利用生物测定方法,连续3年跟踪调查各生育期土壤微生物数量及土壤酶活性变化,旨在明确转基因和非转基因大豆的土壤微环境安全性.试验结果表明,ZH10-6与中黄10通过连续三年比较,不同年份和不同生育期的土壤细菌、真菌、放线菌数量变化势态相似,对微生物数量影响一致且二者之间差异不显著(P>0.05);虽然土壤脲酶、蔗糖转化酶和脱氢酶活性随生长期不同而发生变化,但二者对3种酶活性的影响不存在差异(P>0.05).这说明转外源基因G2-EPSPS和GAT的抗草甘膦ZH10-6与非转基因不抗草甘膦中黄10拥有相似的土壤微环境,具有相同的环境安全性.%Environmental safety assessment can provide scientific data for improving transgenic soybean production development and it has very theoretic and practical meaning. In this study, transgenic soybean ZH10-6 pyramiding two genes of G2-EPSPS and GAT and non-transgenic soybean of the receptor variety (ZH10) were investigated for soil microorganisms and soil enzyme activities in three years using biological methods. i. The results showed that the population sizes of soil bacteria, fungi, actinomycetes during the whole growth period were similar in different years, and no significant difference was found between ZH10-6 and ZH10 (P>0.05). Activities of soil urease, sucralose invertase and dehydrogenase varied among the growth season, but did not differ between ZH10-6 and ZH10 soybean fields (P>0.05). Our results indicated that transgenic soybean ZH10-6 has as the same safety of environment as its receptor of ZH10.
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