转cry1Ie基因玉米残体对赤子爱胜蚓的生长发育及体内酶活性的影响

摘要

Bacillus thuringiensisinsecticidal protein expressed in transgenic corn can be released into soil through root exudates and upon decomposition of the plant residues. The fate of the insecticidal Cry1Ie protein from plant residues of the transgeniccrylIe corn was studied in the soil-dwellingEisenia foetida, by measuring the effects of Bt-corn on growth, development and enzyme activities of the earthwormE. foetida under various trial conditions. Finely ground Bt-corn leaves and stalks added to soil had no deleterious effects on survival and did not result in significant changes in growth and development ofE. fetida. The activities of SOD and POD ofE. fetida in 50B+0N (50 g Bt/500 g soil) group were significant higher than that in 0B+50N (50 g non-Bt/500 g soil) group, suggesting that Bt corn could induce the activities of SOD and POD ofE. fetida within a short time. No differences in protein contents andthe activities of AChE and CAT were detected between treatment groups.%转 Bt 基因抗虫玉米通过根系分泌物或植株残体降解等方式将植株表达的 Bt 杀虫蛋白释放到土壤中。本文通过在人工土壤中添加磨碎的转cry1Ie基因玉米植株残体来饲养赤子爱胜蚓Eisenia foetida，研究了转cry1Ie基因玉米植株残体对赤子爱胜蚓生长发育及体内酶活性的影响。结果显示，在63 d的试验中，添加转 cry1Ie 基因玉米植株残体处理组没有引起赤子爱胜蚓的死亡。5个处理组与对照组中每7 d的蚯蚓体重、产茧数量、茧孵化数量均没有显著差异。SOD酶和POD酶活力随Bt玉米植株残体组织含量的增加而增加，50B＋0N（50 g Bt/500 g土壤）处理组中SOD酶和POD酶活力要显著高于0B＋50N（50 g non-Bt/500 g土壤）对照组。因此，Bt玉米植株残体可以在7 d时诱导赤子爱胜蚓SOD酶和 POD酶活性。但5个处理组与对照组中赤子爱胜蚓的总蛋白含量、AChE和CAT酶活力没有显著的差异。