类群特异性PCR引物设计与评估

摘要

本文以真核藻类18S rDNA类群特异性PCR引物的设计与评估为例,详细介绍了如何利用Primrose等一系列程序设计类群特异性PCR引物并评估其敏感性与特异性,并对这一方法的优势与应用类群特异性PCR引物进行群落多样性分析需要注意的问题进行了讨论.%We take the design and evaluation of eukaryotic algae-targeted 18S rDNA PCR primers as an example,to give a step-by-step illustration of how to design and assess group-targeted PCR primers using Primrose and other programs.Then we summarize the advantages of the design & evaluation pipeline by comparing the amplification efficiency of newly designed and previously reported 18S rDNA universal primers.Cautions are also emphasized on using PCR-based strategies to assess community diversity.