四君子汤合当归补血汤对荷瘤小鼠化疗后免疫功能的影响

摘要

目的：观察四君子汤合当归补血汤对环磷酰胺化疗荷瘤小鼠的免疫保护作用。方法建立小鼠肉瘤S180移植性肿瘤模型，24 h后随机分为5组：荷瘤对照组、环磷酰胺组（cyclophosphamide，CTX）、四君子汤合当归补血汤大中小3个剂量组。荷瘤对照组给予生理盐水腹腔注射0.2 mL/（只·d），生理盐水灌胃0.4 mL/（只·d）；环磷酰胺组给予腹腔注射CTX 20 mg/（kg·d），生理盐水灌胃0.4 mL/（只·d）；四君子汤合当归补血汤小剂量组给予CTX腹腔注射20 mg/（kg·d），中药灌胃0.1 g/（只·d）；四君子汤合当归补血汤中剂量组给予CTX腹腔注射20 mg/（kg·d），中药灌胃0.2 g/（只·d）；四君子汤合当归补血汤大剂量组给予CTX腹腔注射20 mg/（kg·d），中药灌胃0.4 g/（只·d）。每日用药1次，10 d后，计算脾脏及胸腺指数，测定NK细胞活性，检测T淋巴细胞增殖能力。结果四君子汤合当归补血汤3个剂量组均能在一定程度上提高化疗荷瘤小鼠的脾脏胸腺指数、NK细胞活性及T淋巴细胞增殖能力，尤其是中剂量组和大剂量组效果明显，与单纯化疗组比较差异有统计学意义（P＜0.05，P＜0.01）。结论四君子汤合当归补血汤能拮抗化疗引起的小鼠机体免疫功能下降。%Objective To explore the protective effect of Sijunzi decoction and Danggui Buxue decoction on the immunological function in tumor-bearing mice treated by cyclophosphamide.Methods Transplantable carcinomas animal model were constructed by inoculating tumor cell to mice for 24 hours,and randomly divided into five groups.Tumor-bearing control group were reveived normal saline 0.2 mL per mice one day by intraperitoneal injection,and normal saline 0.4 mL per mice each day by intragastric administration.Cyclophosphamide group were reveived CTX 20 mg per kg weight each day,and normal saline 0.4 mL per mice each day.Sijunzi decoction and Danggui Buxue Decoction low dose group were reveived CTX 20 mg per kg weight each day and traditional Chinese medicine 0.1 g per mice each day.Sijunzi decoction and Danggui Buxue Decoction mediate dose group were reveived CTX 20 mg per kg weight each day and traditional Chinese medicine 0.2 g per mice each day.Sijunzi decoction and Danggui Buxue Decoction high dose group were reveived CTX 20 mg per kg weight each day and traditional Chinese medicine 0.4 g per mice each day.The weight indices of thymus gland and spleen,NK-cell activity and the proliferation of T-lymphocyte in five groups were measured after ten 10 days treatment.Results The weight indices of thymus gland and spleen,NK-cell activity and the proliferation of T- lymphocyte were increased in all three Sijunzi decoction and Danggui Buxue decoction groups,especially in Sijunzi decoction and Danggui Buxue decoction mediate and high dose group,the differences between them and CTX group were statistically significan(P<0.05,P<0.01).Conclusion Sijunzi decoction and Danggui Buxue decoction can antagonize the decrease of immunological function in tumor-bearing mice treated by chemotherapy.