新疆厚叶岩白菜素提取方法优化及不同部位含量的研究

摘要

目的：对新疆厚叶岩白菜素进行提取方法优化及不同部位含量的测定，为高效提取厚叶岩白菜素提供依据。方法利用正交实验，对醇提法的乙醇浓度、乙醇用量、提取时间、提取次数4个因素进行综合考虑、筛选，以及对厚叶岩白菜素不同部位进行含量测定。结果优选出最佳的乙醇提取新疆厚叶岩白菜素工艺条件为8倍量50％的乙醇，回流提取3次，每次0.5 h。厚叶岩白菜不同部位的岩白菜素差异具有统计学意义（P<0.05）。主根和须根中岩白菜素的含量较高，达到8.65%～9.58%，而叶中岩白菜素的含量相对较低，仅为0.15%。厚叶岩白菜素嫩叶含量显著高于老叶。结论本实验研究对高效提取新疆厚叶岩白菜素提供了有力的技术支撑，同时为新疆厚叶岩白菜药材质量评价奠定了理论基础。%Objective To extraction method optimization and the content of the different parts of Xinjiang Bergenia crassifolia (L.), provide the basis for efficient extraction of Bergenia crassifolia pigment. Method Using the orthogonal experiment, the alcohol extraction process of ethanol concentration, dosage of ethanol, extraction time, extraction times, as well as to Bergenia crassifolia pigment content of the different part. Results Of optimization, the best ethanol extract of xinjiang Bergenia crassifolia pigment process conditions is 8 times the amount of 50%ethanol, extracting 3 times, each time 0.5 h. In Bergenia crassifolia pigment content of different parts exists obvious difference. Bergenia crassifolia pigment content of Taproot and fibrous root is higher, at 8.65%to 9.58%, while Bergenia crassifolia pigment content of the leaves is relatively low, at just 0.15%. Bergenia crassifolia pigment content of leaves significantly higher than the old leaves. Conclusion This experimental study on efficient extraction of xinjiang Bergenia crassifolia pigment to provide strong technical support and theoretical basis.