炒艾叶与艾叶炭中桉油精含量的考察

摘要

Objective To improve the quality of aiye processed products, an eucalyptol content in commercially available aiye two processed products of chao aiye and aiye tan was investigated.Methods A capillary gas chromatography was used.The sample was prepared with n-hexane by reflux condensation.Chromatographic conditions: The separation was carried on an Ailgent DB-1 capillary column(30 mm ×0.320 mm ×0.25 μm). Inlet temperature was 200℃ and FID temperature was 250℃.The programmed column temperature was set as follows:maintained at 100℃ for 6 min and raised to 160℃ at the rate of 20℃/min followed by holding for 3min.The splitting-ratio was 5.0:1.The carried gas was nitrogen, flow rate was 1.0 mL/min.Injection volume was 1μL.Results In the given chromatographic conditions, the eucalyptol chromatographic separation had good, and the separation degree was greater than 1.5 between eucalyptol and other impurity peak.The linear range of eucalyptol was 11.4-114.0 mg/mL(r=0.999 5). Methods repeatability and recovery were good.The minimum limit of quantification was 0.5μg/mL.The results of determination of eucalyptol show that the eucalyptol content in the commercially available 11 batch of chao aiye was between 5.6-78.2 μg /g, and 12 batch of aiye tan had no eucalyptol. Conclusion The processing technology of current commercially available aiye processed products of chao aiye and aiye tan need to be improved, and the quality standard need to be improved.%目的 为了提高艾叶炮制品质量,对市售艾叶2个炮制品炒艾叶与艾叶炭中桉油精含量进行考察.方法 采用毛细管气相色谱法,用正已烷回流提取样品;色谱条件:以Agilent DB-1甲基硅橡胶石英毛细管柱(30 m ×0.320 mm ×0.25μm)为色谱柱,进样口温度200℃,氢火焰离子化检测器(flame ionization detector, FID)温度250℃;程序升温,起始温度为100℃,保持6 min,再以20℃/min的速率升至160℃,保持3 min;分流进样,分流比5.0:1;载气为氮气,流速1.0 mL/min;进样量1μL.结果 在给定的色谱条件下,桉油精分离良好,与其它杂质峰分离度大于1.5;桉油精在11.4~114.0μg/mL之间与峰面积呈良好的线性关系,方法重复性和加样回收率均好,最小定量限0.5μg/mL.通过对桉油精含量测定,表明市售9家中药饮片厂生产的11批炒艾叶中桉油精含量在5.6~78.2μg/g之间,12批艾叶炭桉油精含量均未检出.结论 目前市售艾叶炮制品炒艾叶、艾叶炭的炮制工艺有待进一步研究改进,需要提高质量标准.