脐血单个核细胞颈内动脉输注对血管性痴呆大鼠认知功能及脑组织神经生长因子的影响

摘要

目的 观察颈内动脉输注脐血单个核细胞(human cord blood mononuclear cells,HCMNCs)对血管性痴呆(vascular dementia,VD)大鼠认知功能及脑组织神经生长因子(nerve growth factor,NGF)含量的影响.方法 改良Pulsinellis四血管阻断法建立VD大鼠模型;体外分离HCMNCs,术后24 h颈内动脉输注数量为3×106/0.5ml的BrdU标记细胞;利用穿梭箱系统和ELISA法检测注射HCMNCs后2,4,8周VD大鼠学习记忆能力以及脑组织NGF含量的变化.结果 模型组大鼠AAR比率(55.4±4.5,42.1±4.5,44.2±3.6)明显低于对照组(91.7±3.9,90.0±4.3,92.5±5.0)(P<0.01),治疗组(67.1±3.3,69.2±4.7,70.8±4.7)较模型组显著提高(P<0.01).术后2周模型组大鼠脑组织NGF含量较对照组明显增高(P<0.01),4周时达到高峰(P<0.01),8周时则明显下降,与2周时相比差异有显著性(P<0.05);颈内动脉输注HCMNCs后治疗组大鼠脑组织NGF含量较模型组显著升高(P<0.01),4周时最高(P<0.01),8周时略有下降,但仍维持在较高水平,与4周时相比差异无显著性(P0.05).结论 颈内动脉输注HCMNCs可显著改善VD大鼠学习记忆能力,增加VD大鼠脑组织NGF含量,具有脑保护作用.%Objective To explore the effect of intracarotid administration of human cord blood mononuclear cells(HCMNCs) on cognitive function and contents of nerve growth factor(NGF) in the vascular dementia (VD) rats. Methods The VD rat model was established by modified pulsinellis 4-Vessel occlusion(4 VO). HCMNCs were isolated from human umbilical cord blood in vitro. The treatment group received intracarotid infusion 3×106/0.5 ml HCMNCs which were prelabelled with BrdU in vitro. The learning-memory abilities and the contents of NGF were observed at 2 w ,4 w and 8 w by computerized shuttle-training ease and Enzyme-linked immunoadsordent assay (ELISA). Results AAR ratio in the model group (55.4±4.5,42.1±4.5,44.2±3.6) showed significantly decrease compared with the control(91.7±3.9,90.0±4.3,92.5±5.0) (P<0.01), and the treatment(67.1±3.3,69.2±4.7,70.8±4.7) was significant higher than the model (P<0.01). The contents of NGF in the model group was significantly higher than that in the control at 2 w, increased to the highest level at 4 w, and then decreased at 8 w, but compared with that at 2 w, significant difference was found. Compared with the model group,the contents of NGF in the treatment showed significantly increase after infusion with HCMNCs, increased to the highest level at 4 w, and then decreased at 8 w but maintain the high level, there were no significant difference between that at 4 w and at 8 w. Conclusion Intraearotid administration of HCMNCs significantly improved the learning-memory abilities and increased the contents of NGF in the VD rats.